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PerCP Mouse Anti-Human CD19 (Leu™-12)
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B4; B-lymphocyte antigen CD19; Leu-12; Leu12
Mouse BALB/c IgG1, κ
Human Chronic Lymphocytic Leukemia (CLL) Cells
Flow cytometry
6.25 μg/mL
20 μL
II B43
Phosphate buffered saline with gelatin and 0.1% sodium azide.

Preparation and Storage

The monoclonal antibody is supplied as 50 μg purified immunoglobulin in 2.0 mL (25 μg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 50 μg in 2.0 mL (25 μg/mL). The PE conjugate is supplied as 25 μg/mL in 1.0 mL. The PerCP conjugate is supplied as 12.5 μg/mL in 2.0 mL (6.25 μg/mL). PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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The CD19 antibody, clone 4G7, is derived from hybridization of P3-X63-Ag8.653 mouse cells with spleen cells from BALB/c mice immunized with human chronic lymphocytic leukemia (CLL) cells.

The CD19 (Leu-12) antibody recognizes a human B-lymphocyte antigen, with a molecular weight of 90 kilodaltons (kDa).

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PerCP dye is part of the BD blue family of dyes. This dye is a fluorescent protein complex with an excitation maximum (Ex Max) of 481 nm and an emission maximum (Em Max) at 675 nm. PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405 nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
481 nm
675 nm
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Development References (8)

  1. Bloomfield C, Gajl-Peczalska K, Frizzera G, Kersey J, Goldman A. Clinical utility of surface markers combined with Lukes-Collins histologic classification in adult lymphoma. N Eng J Med. 1979; 301:512. (Biology).
  2. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:34-36.
  3. Foucar K, Goeken JA. Clinical application of immunologic techniques to the diagnosis of lymphoproliferative and immunodeficiency disorders. Lab Med. 1982; 13:403-413. (Biology).
  4. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood. 1987; 70(5):1316-1324. (Biology). View Reference
  5. Meeker TC, Miller RA, Link MP, Bindl J, Warnke R, Levy R. A unique human B lymphocyte antigen defined by a monoclonal antibody.. Hybridoma. 1984; 3(4):305-20. (Biology). View Reference
  6. Moldenhauer G, Dörken B, Schwartz R, Pezzutto A, Knops J, Hammerling GJ. Analysis of ten B lymphocyte-specific workshop monoclonal antibodies. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:61-67.
  7. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
  8. Warnke RA, Link MD. Identification and significance of cell markers in leukemia and lymphoma. Ann Rev Med. 1983; 34:117. (Biology).
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures. 


Although not required, these products are manufactured in accordance with Good Manufacturing Practices.