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PE-Cy™7 Mouse Anti-Human CD2
製品詳細
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BD™
LFA-2; Lymphocyte function antigen-2; T11/Leu-5; Rosette receptor
Human
Mouse BALB/c IgG2a, κ
CD3-activated Human T Lymphocytes
Flow cytometry
50 μg/mL
5 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation and Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

335786 Rev. 1
抗体の詳細
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L303.1

• CD2, clone S5.2, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T lymphocytes activated by mixed lymphocyte culture.

• CD2, clone L303.1, is derived by the fusion of Sp2/0 myeloma cells with splenocytes from BALB/c mice immunized with CD3-activated human T lymphocytes.

CD2 recognizes a human lymphocyte antigen, 45 to 50 kilodaltons (kDa), which also forms the binding site for sheep erythrocytes.

335786 Rev. 1
フォーマットの詳細
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PE-Cy7
PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE-Cy7
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
781 nm
335786 Rev.1
引用&参考文献
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View product citations for antibody "335786" on CiteAb

Development References (9)

  1. Lanier LL, Phillips JH. A map of the cell surface antigens expressed on resting and activated human natural killer cells. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:157-170.
  2. Bieber CP, Howard FD, Pennock J, Wong J, Shorthouse R, Stinson EB. Preparation, characterization, and primate testing of monoclonal antithymocyte globulin.. Transplantation. 1981; 31(4):283-9. (Biology). View Reference
  3. Bierer BE, Peterson A, Gorga JC, Herrmann SH, Burakoff SJ. Synergistic T-cell activation via the physiological ligands for CD2 and the T-cell receptor. J Exp Med. 1988; 168:1145-1156. (Biology).
  4. Crawford K, Gabuzda D, Pantazopoulos V, et al. Circulating CD2+ monocytes are dendritic cells.. J Immunol. 1999; 163(11):5920-8. (Biology). View Reference
  5. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
  6. Howard FD, Ledbetter JA, Wong J, Bieber CP, Stinson EB, Herzenberg LA. A human T lymphocyte differentiation marker defined by monoclonal antibodies that block E-rosette formation.. J Immunol. 1981; 126(6):2117-22. (Biology). View Reference
  7. Koyasu S, Lawton T, Novick D, et al. Role of interaction of CD2 molecules with lymphocyte function-associated antigen 3 in T-cell recognition of nominal antigen.. Proc Natl Acad Sci USA. 1990; 87(7):2603-7. (Biology). View Reference
  8. MacDonald KP, Munster DJ, Clark GJ, Dzionek A, Schmitz J, Hart DN. Characterization of human blood dendritic cell subsets.. Blood. 2002; 100(13):4512-20. (Biology). View Reference
  9. Moingeon P, Chang H, Wallner BP, Stebbins C, Frey AZ, Reinherz EL. CD2-mediated adhesion facilitates T-lymphocyte antigen-recognition function. Nature. 1989; 339:312-314. (Biology).
すべて表示する (9) 表示項目を減らす
335786 Rev. 1

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