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      FITC Mouse Anti-Human CD45RA
      製品詳細
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      BD™
      CD45RA; CD45R
      Human
      Mouse BALB/c IgG1, κ
      Low-buoyant–density human lymphocytes
      Flow cytometry
      50 μg/mL
      20 μL
      5788
      Phosphate buffered saline with gelatin and 0.1% sodium azide.
      RUO (GMP)


      Preparation and Storage

      The monoclonal antibody is supplied as 50 μg of purified immunoglobulin in 2.0 mL (25 μg/mL) of phosphate buffered saline (PBS). The FITC conjugate is supplied as 100 μg in 2.0 mL (50 μg/mL) of PBS. The PE-Cy7 conjugate is supplied as 25 μg in 0.5 mL (50 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

      Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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      347723 Rev. 1
      抗体の詳細
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      L48

      The CD45RA antibody, clone L48, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with low-buoyant–density human lymphocytes.

      The CD45RA antibody recognizes a 220-kilodalton (kDa) isoform of the leucocyte common antigen (LCA). The CD45RA antigen is a member of the CD45 antigen family that also includes the CD45, CD45RB, and CD45RO antigens.

      347723 Rev. 1
      フォーマットの詳細
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      347723 Rev.1
      引用＆参考文献
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      Development References (11)

      1. Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:788-803.
      2. Gamadia LE, Rentenaar RJ, Baars PA, et al. Differentiation of cytomegalovirus-specific CD8(+) T cells in healthy and immunosuppressed virus carriers.. Blood. 2001; 98(3):754-61. (Biology). View Reference
      3. Ledbetter JA, Rose LM, Spooner CE, Beatty PG, Martin PJ, Clark EA. Antibodies to common leukocyte antigen p220 influence human T cell proliferation by modifying IL 2 receptor expression.. J Immunol. 1985; 135(3):1819-25. (Biology). View Reference
      4. Mackus WJ, Frakking FN, Grummels A, et al. Expansion of CMV-specific CD8+CD45RA+CD27- T cells in B-cell chronic lymphocytic leukemia.. Blood. 2003; 102(3):1057-63. (Biology). View Reference
      5. Maldonado A, Mueller YM, Thomas P, Bojczuk P, O'Connors C, Katsikis PD. Decreased effector memory CD45RA+ CD62L- CD8+ T cells and increased central memory CD45RA- CD62L+ CD8+ T cells in peripheral blood of rheumatoid arthritis patients.. Arthritis Res Ther. 2003; 5(2):R91-6. (Biology). View Reference
      6. Morimoto C, Letvin NL, Distaso JA, Aldrich WR, Schlossman SF. The isolation and characterization of the human suppressor/inducer T-cell subset. J Immunol. 1985; 134:1508-1515. (Biology).
      7. Plaeger-Marshall S, Hultin P, Bertolli J, et al. Activation and differentiation antigens on T cells of healthy, at-risk, and HIV-infected children.. J Acquir Immune Defic Syndr. 1993; 6(9):984-93. (Biology). View Reference
      8. Rose LM, Ginsberg AH, Rothstein TL, Ledbetter JA, Clark EA. Selective loss of a subset of T helper cells in active multiple sclerosis.. Proc Natl Acad Sci USA. 1985; 82(21):7389-93. (Biology). View Reference
      9. Serra HM, Krowka JF, Ledbetter JA, Pilarski LM. Loss of CD45R (Lp220) represents a post-thymic T cell differentiation event.. J Immunol. 1988; 140(5):1435-41. (Biology). View Reference
      10. Sobel RA, Hafler DA, Castro EE, Morimoto C, Weiner HL. The 2H4 (CD45R) antigen is selectively decreased in multiple sclerosis lesions.. J Immunol. 1988; 140(7):2210-4. (Biology). View Reference
      11. Walker EB, Haley D, Miller W, et al. gp100(209-2M) peptide immunization of human lymphocyte antigen-A2+ stage I-III melanoma patients induces significant increase in antigen-specific effector and long-term memory CD8+ T cells.. Clin Cancer Res. 2004; 10(2):668-80. (Biology). View Reference
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      347723 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

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