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Antibody Cocktail (IFN-γ FITC, CD69 PE, CD4 PerCP-Cy™5.5, CD3 APC)

Antibody Cocktail (IFN-γ FITC, CD69 PE, CD4 PerCP-Cy™5.5, CD3 APC)

(RUO (GMP))
製品詳細
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BD FastImmune™
Human
Flow cytometry
RUO (GMP)
Phosphate buffered saline with BSA, beta-lactoglobulin, and 0.1% sodium azide.


説明

Anti-Hu–IFN-γ, clone 25723.11, is derived from the hybridization of mouse P3X-63- Ag8.653 myeloma cells with lymph node cells from BALB/c mice immunized with recombinant human IFN-γ.

CD69, clone L78, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line.

CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immununized with human peripheral blood T lymphocytes.

CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

Anti-Human Interferon- γ (Anti-Hu–IFN-γ) recognizes a 20- to 25-kilodalton (kd) glycoprotein.

CD69 recognizes a very early human activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28- and 32-kd chains that are held together by disulfide bridges.

The CD4 antigen, Mr 55 kd, is present on T-helper/inducer lymphocytes and monocytes.

CD3 reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kd.

The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).


Anti-Hu–IFN-γ, clone 25723.11, is derived from the hybridization of mouse P3X-63- Ag8.653 myeloma cells with lymph node cells from BALB/c mice immunized with recombinant human IFN-γ.

CD69, clone L78, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line.

CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immununized with human peripheral blood T lymphocytes.

CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

Anti-Human Interferon- γ (Anti-Hu–IFN-γ) recognizes a 20- to 25-kilodalton (kd) glycoprotein.

CD69 recognizes a very early human activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28- and 32-kd chains that are held together by disulfide bridges.

The CD4 antigen, Mr 55 kd, is present on T-helper/inducer lymphocytes and monocytes.

CD3 reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kd.

The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).

調製と保管

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

337184 Rev. 1
構成品
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説明 クローン アイソタイプ EntrezGene ID
CD3 APC SK7 IgG1, κ N/A
IFN-γ FITC TgMab-2 IgG1, κ N/A
CD69 PE 2D7/Basophils IgG1, κ N/A
CD4 PerCP-CY5.5 SK3 IgG1, κ N/A
337184 Rev. 1
引用&参考文献
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開発者向け参考資料 (39)

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337184 Rev. 1

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