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BD Simultest™
Flow cytometry
Buffered saline with gelatin and 0.1% sodium azide.


BD Simultest™ Leucogate™ (CD45/CD14) is a two-color direct immunofluorescence reagent for establishing an optimal lymphocyte gate for immunophenotyping of erythrocyte-lysed whole blood (LWB). BD Simultest Leucogate is for in vitro diagnostic use with BD in vitro diagnostic reagents. See the appropriate BD in vitro diagnostic package insert for additional information.


• For in vitro diagnostic use.

• When stored at 2° to 8°C, the antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date.

• The antibody reagent should not be frozen or exposed to direct light during storage or during incubation with cells. Keep the reagent vial dry.

• Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.

• The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.

340040 Rev. 1
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説明 クローン アイソタイプ EntrezGene ID
CD45 FITC 2D1 IgG1, κ N/A
CD14 PE MΦP9 IgG2b, κ 929
340040 Rev. 1
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開発者向け参考資料 (16)

  1. Bernard A, Boumsell L, Hill C. Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman S, ed. Leucocyte Typing. New York, NY: Springer-Verlag; 1984:82-108.
  2. Bernstein ID, Self S. Joint report of the Myeloid Section of the Second International Workshop on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human Myeloid and Hematopoietic Cells. New York, NY: Springer-Verlag; 1986:1-25.
  3. Beverley P. Touraine J, Trager J, Betuel H, ed. Transplantation and Clinical Immunology XI. 1980:87-94.
  4. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes. NCCLS document H42-T. 1992. (Biology).
  5. Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:788-803.
  6. Dimitriu-Bona A, Burmester GR, Waters SJ, Winchester RJ. Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol. 1983; 130(1):145-152. (Biology). 参考文献を見る
  7. Giorgi JV. Lymphocyte subset measurements: significance in clinical medicine. In: Rose NR, Friedman H, Fahey JL, ed. Manual of Clinical Laboratory Immunology. 3rd ed.. Washington, DC: American Society for Microbiology; 1986:236-246.
  8. Goyert S, Tesio L, Ashman L, et al. Knapp W, Dörken B, Gilks WR, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:789-794.
  9. Herrmann F, Komischke B, Odenwald E, Ludwig W. Use of monoclonal antibodies as a diagnostic tool in human leukemia, I: Acute myeloid leukemia and acute phase of chronic myeloid leukemia. Blut. 1983; 47:157-163. (Biology).
  10. Jackson A. Basic phenotyping of lymphocytes: selection and testing of reagents and interpretation of data. Clin Immunol Newslett. 1990; 10:43-55. (Biology).
  11. Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
  12. Jayaram Y, Hogg N. Surface expression of CD14 molecules on human neutrophils. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:796-797.
  13. Loken MR, Brosnan JM, Bach BA, Ault KA. Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry. Cytometry. 1990; 11(4):453-459. (Biology). 参考文献を見る
  14. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture–Fourth Edition; Approved Standard. NCCLS document H3-A4. 1998. (Biology).
  15. Rowe D, Beverley P. Characterisation of breast cancer infiltrates using monoclonal antibodies to human leucocyte antigens. Br J Cancer. 1984; 49:149-159. (Biology).
  16. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
すべて表示する (16) 表示項目を減らす
340040 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.