-
Your selected country is
Japan
- Change country/language
-
抗体試薬
- フローサイトメトリー用試薬
-
ウェスタンブロッティング抗体試薬
- イムノアッセイ試薬
-
シングルセル試薬
- BD® AbSeq Assay | シングルセル試薬
- BD Rhapsody™ Accessory Kits | シングルセル試薬
- BD® Single-Cell Multiplexing Kit | シングルセル試薬
- BD Rhapsody™ Targeted mRNA Kits | シングルセル試薬
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit | シングルセル試薬
- BD Rhapsody™ TCR/BCR Profiling Assays (VDJ Assays) | シングルセル試薬
- BD® OMICS-Guard Sample Preservation Buffer
-
細胞機能評価のための試薬
-
顕微鏡・イメージング用試薬
-
細胞調製・分離試薬
-
- BD® AbSeq Assay | シングルセル試薬
- BD Rhapsody™ Accessory Kits | シングルセル試薬
- BD® Single-Cell Multiplexing Kit | シングルセル試薬
- BD Rhapsody™ Targeted mRNA Kits | シングルセル試薬
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit | シングルセル試薬
- BD Rhapsody™ TCR/BCR Profiling Assays (VDJ Assays) | シングルセル試薬
- BD® OMICS-Guard Sample Preservation Buffer
- Japan (Japanese)
- Change country/language
Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Death Staurosporine
Induction of Apoptosis by Treatment with Staurosporine
Staurosporine ( Streptomyces staurospores) is a relatively non-selective protein kinase inhibitor, which blocks many kinases to different degrees. Staurosporine is often used as a general method for inducing apoptosis 1.
Protocol for Staurosporine-induced apoptosis:
- Add 1 µM (final concentration) staurosporine (eg, Sigma S6942) to cell suspension (e.g., 5 x 10 5 cells/ml in tissue culture medium).
- Perform a time course to obtain optimum results; a 1-6 hr incubation at 37°C is suggested. *Note: some cells or cell lines may take up to 12 hours or more to induce apoptosis.
- Proceed with assays designed to evaluate induction of apoptosis.
Kabir J, Lobo M, Zachary I. 2002 Staurosporine induces endothelial cell apoptosis via focal adhesion kinase dephosphorylation and focal adhesion disassembly independent of focal adhesion kinase proteolysis. J. Biochem. 367: 145-155.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.