Flow cytometric analysis of human CCR10 expression on peripheral blood lymphocytes. Whole blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. The leucocytes were then stained with Alexa Fluor® 488 Mouse Anti-Human CD3 antibody (Cat No. 557694) and either PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648; Left Panel) or PE Mouse Anti-Human CCR10 (Cat. No. 563656; Right Panel). The two-color flow cytometric dot plot shows the correlated expression patterns of CD3 versus CCR10 (or Ig Isotype control staining) for gated events with the forward and side light-scatter characteristics of viable peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
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Flow cytometric analysis of human CCR10 expression on peripheral blood lymphocytes. Whole blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. The leucocytes were then stained with Alexa Fluor® 488 Mouse Anti-Human CD3 antibody (Cat No. 557694) and either PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648; Left Panel) or PE Mouse Anti-Human CCR10 (Cat. No. 563656; Right Panel). The two-color flow cytometric dot plot shows the correlated expression patterns of CD3 versus CCR10 (or Ig Isotype control staining) for gated events with the forward and side light-scatter characteristics of viable peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
C-C CKR-10, C-C CKR-10, G-protein coupled receptor 2, CCR-10, GPR2
Human (QC Testing)
Mouse C57BL/6 IgG2a, κ
Human CCR10 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738351
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
PE Mouse IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554648
Alexa Fluor® 488 Mouse Anti-Human CD3 RUO
Size
100 Tests
Cat No.
557694
563656 Rev. 2
Antibody Details
1B5
The 1B5 monoclonal antibody specifically binds to CCR10 (CC-chemokine receptor 10). CCR10 is a seven transmembrane domain G-protein-coupled receptor that is also known as GPR-2 (G-protein coupled receptor 2). CCR10 is expressed by cells that mediate epithelial immunity including a small subset of T memory cells, IgA-secreting B cells, dermal endothelial cells and fibroblasts. It is also expressed on EBV-immortalized B cells. Chemokines CCL27 and CCL28 are the ligands for CCR10. CCR10 reportedly not only functions in regulating the homing and function of cells that mediate epithelial immunity. It may also regulate the epithelial localization, survival, and proliferation of tumor cells and facilitate their evasion from immune surveillance.
563656 Rev. 2
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
563656 Rev.2
Citations & References
Development References (6)
-
Homey B, Wang W, Soto H, et al. Cutting edge: the orphan chemokine receptor G protein-coupled receptor-2 (GPR-2, CCR10) binds the skin-associated chemokine CCL27 (CTACK/ALP/ILC). J Immunol. 2000; 164(7):3465-3470. (Biology). View Reference
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Jaimes MC, Rojas OL, Kunkel EJ, et al. Maturation and trafficking markers on rotavirus-specific B cells during acute infection and convalescence in children. J Virol. 2004; 78(20):10967-10976. (Clone-specific). View Reference
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Marchese A, Docherty JM, Nguyen T, et al. Cloning of human genes encoding novel G protein-coupled receptors. Genomics. 1994; 23(3):609-618. (Biology). View Reference
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Pan J, Kunkel EJ, Gosslar U, et al. A novel chemokine ligand for CCR10 and CCR3 expressed by epithelial cells in mucosal tissues. J Immunol. 2000; 165(6):2943-2949. (Biology). View Reference
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Soler D, Humphreys TL, Spinola SM, Campbell JJ. CCR4 versus CCR10 in human cutaneous TH lymphocyte trafficking. Blood. 2003; 101(5):1677-1682. (Immunogen: Flow cytometry). View Reference
-
Xiong N, Fu Y, Hu S, Xia M, Yang J. CCR10 and its ligands in regulation of epithelial immunity and diseases. Protein Sci. 2012; 3(8):571-580. (Biology). View Reference
563656 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
