Description
The BD Pharmingen™ BrdU In-Situ Detection Kit is designed for immunohistochemical staining of BrdU in frozen sections, formalin-fixed paraffin-embedded sections, and cultured or isolated cells on slides. Our monoclonal antibody against BrdU works in all species tested (human, mouse, and rat) and provides improved specific staining with minimal background. The directly biotinylated mouse antibody eliminates the need for a species-specific secondary antibody thus allowing it to be used in mouse tissues. The BrdU In-Situ Detection Kit features our BD™ Retrievagen A, an antigen retrieval solution designed specifically to unmask antigenic sites, preserve tissue morphology, and enable simultaneous staining of other surface antigens in conjunction with BrdU. This important feature of the kit enables study of the proliferation state of phenotypically defined cells within the micro-environment of tissues. Consistent results are assured by providing all critical reagents in addition to a comprehensive series of protocols for staining BrdU in paraffin-embedded and frozen tissue sections and cultured cells, and in conjunction with other antigens. Control slides are provided in the kit to serve as a reference.
Preparation And Storage
All the components of the BrdU kit must be stored at 4°C. Some solutions require diluting or mixing prior to use.
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| Biotinylated Anti-BrdU Antibody | 0.5 mL (1 ea) | 51-75512L | N/A |
| Diluent Buffer | 15 mL (1 ea) | 51-2373KD | N/A |
| Retrievagen A, Solution 1 | 10 mL (1 ea) | 51-7540KD | N/A |
| Retrievagen A, Solution 2 | 50 mL (1 ea) | 51-7541KD | N/A |
| Fixation Buffer | 10 mL (1 ea) | 51-2374KD | N/A |
| DAB Chromogen | 0.75 mL (1 ea) | 51-7549KD | N/A |
| DAB Buffer | 10 mL (1 ea) | 51-7548KD | N/A |
| Streptavidin-HRP | 6 mL (1 ea) | 51-7642KD | N/A |
| Control Slides | 5 Slides (1 ea) | 51-7550KD | N/A |
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
