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Antibodies to Human Cell-Surface Markers Tested for BD Phosflow Protocols (Technical Document)

Many surface antigens are sensitive to fixation/permeabilization procedures, resulting in loss of recognition by the antibodies. This is a summary of tested antibodies under several fixation/permeabilization conditions.

Construction of Stem Cell-Derived Hepatic Grafts Using Organ Engineering Technology (References)

Our goals are to develop a natural hepatic platform for repopulation with human embryonic or induced pluripotent stem (ES,iPS) cell-derived liver cells to engineer functional transplantableliver grafts.

Cardiovascular Regeneration by Endocardial-Derived Cardiac Progenitors (References)

We have demonstrated evidence for the presence of such multipotent progenitors in the endocardial cushions in developing mouse and human hearts.

Arming Cytotoxic Cells with Chimeric Antigen Receptors (CARs) for Treatment of Refractory Cancer: Pr (References)

To investigate and determine the best cell source for the generation of CAR bearing NK cells for the treatment of hematological cancer

Effect of Maternal Metabolic Intrauterine Environment on Fetal Methylome (References)

To explore whether environmental insults leave a distinguishable "epigenetic footprint" on the fetus

A Marker Toolbox for Neural Stem Cell Purification (References)

Here we propose to overcome this technical hurdle by developing a set of markers that can definitively identify neural stem/progenitor cells in the mammalian brain.

The Derivation of Oligodendrocyte Progenitors from Shiverer and Wild-Type Mouse Embryonic Stem Cells (References)

Stem cell based therapies have become increasingly popular due to their ability to not only replace lost cell types but also aid in regeneration and recovery of function in hopes of improving one or more of these issues.

Induction of Pluripotency by CRISPER-Cas9 Epigenetic Engineering (References)

Using a Cas9-based trans-activator targeted to DNA sequences by gRNA molecules in eukaryotic cells will allow induction of specific expression of endogenous target genes that could lead to reprogramming of the cells

Identification of Brown Fat Precursor Cells (References)

The goal of this project is to 1) identify a cell population that retains a potency to differentiate into brown fat cells in WAT and 2) characterize what defines the self-renewal, maintenance and differentiation of precursors.

Human Glioblastoma Stem Cells and GSC-derived Endothelial Cells: Identification of Novel Markers (References)

To identify new molecules expressed in the cell surface proteome of GSCs and EC-GSCs that could allow defined strategies for the isolation of targeted cells from heterogeneous cell tumor populations

Role of ATM Kinase in Breast Cancer Stem Cell Targeting (References)

To support a grant project by an extensive flow cytometry analysis of pathways that are deregulated when we downregulate ATM in breast cancer stem cells

Identification of Human Lung Cancer Stem Cell Markers (References)

We aim to identify novel cell surface markers for isolating lung CSCs, which is essential for characterization of these important cells and holds the promise of prognostic tests and therapeutic interventions for this disease.

Instrument Setup Guide for BD OneFlow Assays (Application Note)

The BD OneFlow™ system provides a comprehensive set of reagents and protocols to reproducibly set up the cytometer and stain patient specimens. The consistent instrument setup and sample staining enable you to acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations in a manner compatible with that prescribed by the EuroFlow™ Consortium.

BD™ FC Beads 8-Color Kit for BD OneFlow™ Assays (Manual)

BD™ FC beads 8-color kit for BD OneFlow™ assays (BD FC beads) are intended to allow software to determine spillover values (SOVs) for fluorescencecompensation.

BD OneFlow LST Application Guide (Application Note)

The BD OneFlow™ system provides a comprehensive set of reagents and protocols to reproducibly set up the flow cytometer and stain patient specimens. The consistent instrument setup and sample staining enable you to acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations in a manner compatible with that prescribed by the EuroFlow™ Consortium.

BD OneFlow™ Solution: Leukemia and lymphoma immunophenotyping (Brochure)

Built on the research and validation work of the EuroFlow™ Consortium for the characterization of hematological malignancies for improved diagnostic outcomes, the BD OneFlow™ solution brings the standardization of leukemia and lymphoma immunophenotyping one step forward, maximizing laboratory efficiency and increasing reliability and accuracy of results for better clinical decisions for each individual’s outcome.

BD FACSCelesta Flow Cytometer (Brochure)

The BD FACSCelesta™ flow cytometer is designed to make multicolor flowcytometry more accessible and allow researchers to benefit from newinnovations in instrument and reagent technology.

Antibodies in EuroFlow™ panels (Tool)

Antibodies in EuroFlow™ panels. Alternative tested single vial markers are included.

BD Biosciences antibodies in EuroFlow multicolor panels (Product Info Sheet)

BD Biosciences and EuroFlow are continuously collaborating to expand the choice of single color reagents with new tested alternatives showing comparable staining patter to the corresponding EuroFlow reference reagents.

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