Tumor Macrophage Polarization (References)
Macrophages are a critical component of anti-tumor immunity but may be subverted from the classically-activated, or M1 phenotype, which mediates tumor elimination, to an alternatively-activated, M2 phenotype.
Role of SOCS in Treg Function (References)
Specific aim 1: Characterize the thymic development and peripheral survival of Tregs in SOCS-1 gene deficient (KO) mice. Specific aim 2: Assess the capacity of Tregs present in SOCS-1 KO mice to regulate immune responses.
Surface Proteins in Stem Cells (References)
Regenerative medicine, through the use of stem cells, has the potential to repair the heart after a heart attack and to restore cardiac function in patients with heart failure, presently an incurable condition.
Intracellular Signaling Networks in Inflammation: A Comparison of Three Diseases of Childhood Using (References)
Systemic juvenile idiopathic arthritis (SJIA) is one of the subtypes of juvenile idiopathic arthritis (JIA), is characterized by a combination of systemic inflammatory features andarthritis, and is the most severe form of JIA.
Dissection of Immune Modulation by MUC1/sec or IEP, Which Enables Induction of an Effective Anti-Tum (References)
In our laboratory, isoforms of the MUC1 molecule were investigated, and it was found that expression of a secreted isoform (MUC1/sec) by an aggressive murine mammary tumor resulted in rejection of the tumor in vivo.
The Role of Plasmacytoid Dendritic Cells (References)
Human plasmacytoid dendritic cells (PDCs) arise from cells lacking lineage markers and CD11c, but expressing HLA-DR. PDCs are further identified phenotypically by expression of CD123, the IL-3Rα chain.
B Cell Alternative Receptors (References)
We have found that resting, naïve human B cells do not respond to schistosomal antigens, suggesting thatendogenous B cell receptors do not recognize schistosomes.
Confirmation of our pilot results will lead to the development of a new, inexpensive prognostic kit for CLL by testing for susceptibility to DPP2 inhibition-induced apoptosis.
Early origins of breast cancer are the subject of intense interest. In some cancers, eg.colon cancer, there is a clear stepwise progression with morphologically recognizable intermediates correlated to molecular alterations.
Identification of Osteoclast Progenitor Cells (References)
Regulation of osteoclast function is central to the understanding of bone diseases such as osteoporosis, rheumatoid arthritis, as well as bone metastases of cancers.
Latexin: A New Tumor Suppressor (References)
Latexin (Lxn) is involved in the regulation of proliferation, apoptosis, self-renewal, and subsequently pool size, of normal hematopoietic stem cells (HSCs).
T Cell Differentiation Poster (Chart)
T Cell Differentiation Poster
Screening and Development of Antibodies for Use in Cell-Based Assays Using Immunofluorescence Micros (Application Note)
To facilitate the use of antibodies for imaging applications, BD Biosciences embarked on a screening process using automated imaging to evaluate a large library of monoclonal antibodies for utility in cellular imaging.
This guide provides instructions for acquiring and analyzing samples stained using the BD Stem Cell Enumeration kit on a BD FACSCalibur flow cytometer.
This guide provides instructions for acquiring and analyzing samples stained with the BD™ Stem Cell Enumeration kit on a BD FACSCanto™ II flow cytometer using BD FACSCanto™ clinical software.
BD FACS Lyse Wash Assistant [and Larger Tank Option] Technical Specifications (Technical Document)
The BD FACS™ Lyse Wash Assistant is an automated sample preparation instrument capable of lysing, washing, mixing, and fixing samples that are then ready for analysis on a flow cytometer.
BD Horizon Brilliant Violet Reagents Datasheet (Product Info Sheet)
BD Horizon Brilliant Violet Reagents Datasheet
Caspase-3 Activation: An Indicator of Apoptosis in Image-Based Assays (Application Note)
The understanding of the cellular signaling processes leading to programmedcell death (“apoptosis”) is of the utmost importance in the study of autoimmunediseases such as rheumatoid arthritis, Parkinson’s, and Alzheimer’s disease.
BD Custom Technology Team (Brochure)
To provide clients with tools to advance discovery and development programs, CTT offers a deep knowledge base of advanced methodologies for immunophenotyping, immunogenicity testing, immune function testing, and more.
After completing this module, you will be able to use BD FACS™ Accudrop technology to determine a precise drop delay for sorting on the BD Influx™ cell sorter.