Biotin Mouse Anti-Rat CD134
Clone OX-40 (RUO)
- Brand BD Pharmingen™
- Alternative Name OX-40 Antigen; Cd134; Tnfrsf4
- Concentration 0.5 mg/ml
- Isotype Mouse BALB/c IgG2b, κ
- Reactivity Rat (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Activated rat lymph node cells
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The OX-40 antibody reacts with the 50-kDa OX-40 Antigen (CD134), also known as OX-40 Receptor, on CD4+ T lymphocytes activated in vitro and in vivo. The antigen is a member of the NGFR/TNFR superfamily, which includes low-affinity nerve growth factor receptor, TNF receptors, the Fas antigen, CD137 (4-1BB), CD27, CD30, and CD40. CD134 supplies costimulatory signals for T-cell proliferation and effector functions. While OX-40 mAb is not mitogenic, it does augment some in vitro T-cell responses. It is also reported to block binding of OX-40 Ligand to OX-40 Antigen.
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.