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Purified Mouse Anti-Human CD49d 9F10 RUO

Purified Mouse Anti-Human CD49d 

Clone  9F10   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name Integrin α4 chain; Integrin alpha 4; ITGA4; IA4; alpha 4 subunit of VLA-4
  • Concentration 0.5 mg/ml
  • Isotype Mouse IgG1, κ
  • Reactivity Human (QC Testing)  Rhesus, Cynomolgus, Baboon, Dog, Cow, Sheep, Horse, Cat (Tested in Development) 
  • Application Flow cytometry (Routinely Tested) 
  • Workshop No. V S215
  • Entrez Gene ID 3676 
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The 9F10 monoclonal antibody specifically reacts with the integrin α4 chain, that is expressed as a heterodimer with either of two β integrin subunits, β1 (CD29) or â7. The α4β1 integrin (VLA-4) is expressed on lymphocytes, monocytes, thymocytes, NK cells, and several B- and T-cell lines, and mediates binding to VCAM-1 (CD106) and the CS-1 region of fibronectin. The α4β7 integrin has a similar tissue distribution, except it is found on only a small subpopulation of thymocytes. Integrin α4β7 also binds fibronectin and VCAM-1, and has been shown in the mouse to preferentially bind the mucosal vascular addressin molecule, MAdCAM-1. This antibody is useful for studies of the expression by and function of cells that express α4 chain-containing integrins. This clone cross-reacts with a subset of peripheral blood lymphocytes, monocytes, and some granulocytes of baboon and both rhesus and cynomolgus macaque monkeys. The distribution on leukocytes is similar to that observed with human peripheral blood leukocytes.

Format
  • Format Purified

Other Formats →

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Purified Mouse IgG1 κ Isotype Control RUO
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FITC Goat Anti-Mouse IgG/IgM Polyclonal RUO
0.5 mg Cat No: 555988

Stain Buffer (FBS) RUO
500 mL Cat No: 554656

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  4. An isotype control should be used at the same concentration as the antibody of interest.


  1. Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell. 1993; 74(1):185-195. View reference

  2. Hemler ME, Huang C, Takada Y, Schwarz L, Strominger JL, Clabby ML. Characterization of the cell surface heterodimer VLA-4 and related peptides. J Biol Chem. 1987; 262(24):11478-11485. View reference

  3. Hemler ME, Kassner P, Bodorova J. CD49d cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995; :1617-1618.

  4. Hemler ME. VLA proteins in the integrin family: structures, functions, and their role on leukocytes. Annu Rev Immunol. 1990; 8:365-400. View reference

  5. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989; :1-1182.

  6. Parker CM, Cepek KL, Russell GJ, et al. A family of beta 7 integrins on human mucosal lymphocytes. Proc Natl Acad Sci U S A. 1992; 89(5):1924-1928. View reference

  7. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995; .

  8. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dorries R, ter Meulen V. Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys. Cytometry. 1997; 29(4):351-362. View reference

556634 Rev. 6
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