North America
South America
Australia & New Zealand
Europe
Middle East / Africa
Asia / Pacific
×
×
Purified NA/LE Hamster Anti-Mouse CD49b HMα2 RUO

Purified NA/LE Hamster Anti-Mouse CD49b 

Clone  HMα2   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name Integrin α2 chain
  • Concentration 1.0 mg/ml
  • Isotype Armenian Hamster IgG1, κ
  • Reactivity Mouse (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
    Immunoprecipitation, Blocking (Reported) 
  • Immunogen Mouse colon carcinoma cell line Colon26
  • Entrez Gene ID 16398 
  • Storage Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm filtered. Endotoxin level is ≤0.01 ng/µg of protein.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The HMα2 antibody reacts with integrin α2 chain (CD49b), the 150-kDa transmembrane glycoprotein that non-covalently associates with the integrin β1 subunit (CD29) to form the integrin α2β1 complex known as VLA-2. VLA-2, a receptor for collagen and laminin, is expressed on some splenic CD4+ T lymphocytes and NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, splenic NK cells, epithelial cells, and platelets; but it is not on thymocytes or Peyer's-patch or lymphnode lymphocytes. The expression of VLA-2 is upregulated on lymphocytes in response to mitogens. The HMα2 antibody has been reported to partially block the interaction of T-cell blasts, but not NK cells, with collagen. Purified HMα2 mAb blocks the staining of splenic NK cells by the anti-CD49b/Pan-NK Cells mAb DX5 (Cat. No. 553858, for the PE conjugate). Therefore, mAb HMα2 may be used like the DX5 mAb for identification of NK cells.

Format
  • Format NA/LE

Other Formats →

Suggested Companion Products

Purified NA/LE Hamster IgG1 κ Isotype Control RUO
0.5 mg Cat No: 553968

FITC Mouse Anti-Armenian and Syrian Hamster IgG Cocktail RUO
0.5 mg Cat No: 554011

Resources & Tools
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.


For immunohistochemical staining, we recommend the use of purified anti-rat CD49b mAb Ha1/29, which cross-reacts with mouse, Cat. No. 559987/554998.


  1. Arase H, Saito T, Phillips JH, Lanier LL. Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2). J Immunol. 2001; 167(3):1141-1144. View reference

  2. Chen H, Paul WE. A population of CD62Llow Nk1.1- CD4+ T cells that resembles NK1.1+ CD4+ T cells. Eur J Immunol. 1998; 28(10):3172-3182. View reference

  3. Miyake S, Sakurai T, Okumura K, Yagita H. Identification of collagen and laminin receptor integrins on murine T lymphocytes. Eur J Immunol. 1994; 24(9):2000-2005. View reference

  4. Noto K, Kato K, Okumura K, Yagita H. Identification and functional characterization of mouse CD29 with a mAb. Int Immunol. 1995; 7(5):835-842. View reference

  5. Tanaka T, Ohtsuka Y, Yagita H, Shiratori Y, Omata M, Okumura K. Involvement of alpha 1 and alpha 4 integrins in gut mucosal injury of graft-versus-host disease. Int Immunol. 1995; 7(8):1183-1189. View reference

557017 Rev. 7
Instruments
Reagents
Applications
Sales & Support
BD BioSciences