Purified Hamster IgG2, λ1 Isotype Control
Clone Ha4/8 (RUO)
- Brand BD Pharmingen™
- Alternative Name Anti-KLH
- Concentration 0.5 mg/ml
- Isotype Armenian Hamster IgG2, λ1
ELISA (Routinely Tested)
Isotype control, Flow cytometry (Tested During Development)
- Immunogen Keyhole limpet hemocyanin
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The Ha4/8 antibody is specific for keyhole limpet hemocyanin (KLH). It is useful as an immunoglobulin isotype-matched negative control for immunofluorescent staining.
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at 4°C.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- An isotype control should be used at the same concentration as the antibody of interest.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
This immunoglobulin is useful as an isotype-matched negative control for immunofluorescent staining. Other reported applications include immunohistochemical staining (IHC). For IHC, we recommend the use of purified Ha4/8 mAb in our special formulation for immunohistochemistry, Cat. No. 550345.