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PerCP-Cy™5.5 Rat Anti-Mouse CD107b
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PerCP-Cy™5.5 Rat Anti-Mouse CD107b
Two-color flow cytometric analysis of CD107b expression on mouse peritoneal exudate cells. Mouse peritoneal exudate cells (PEC) were isolated 4 days post-stimulation by intraperitoneal injection of a 3% thioglycollate solution. The PEC were stained with APC Rat Anti-Mouse CD11b (Cat. No. 553312/561690) and either PerCP-Cy™5.5 Rat IgG1, κ Isotype Control (Cat. No. 560537; Left Panel) or PerCP-Cy™5.5 Rat Anti-Mouse CD107b (Cat. No. 564844; Right Panel).  Two-color flow cytometric contour plots showing the correlated expression of CD107b (or Ig Isotype control staining) versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable PEC. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Two-color flow cytometric analysis of CD107b expression on mouse peritoneal exudate cells. Mouse peritoneal exudate cells (PEC) were isolated 4 days post-stimulation by intraperitoneal injection of a 3% thioglycollate solution. The PEC were stained with APC Rat Anti-Mouse CD11b (Cat. No. 553312/561690) and either PerCP-Cy™5.5 Rat IgG1, κ Isotype Control (Cat. No. 560537; Left Panel) or PerCP-Cy™5.5 Rat Anti-Mouse CD107b (Cat. No. 564844; Right Panel).  Two-color flow cytometric contour plots showing the correlated expression of CD107b (or Ig Isotype control staining) versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable PEC. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
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BD Pharmingen™
Mac-3; Lamp2; LAMP-2; Lysosome-associated membrane glycoprotein 2; LGP-B
Mouse (QC Testing)
Rat LEW x BN IgG1, κ
Mouse C57Bl/6 peritoneal exudate cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
16784
AB_2738980
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  5. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
  6. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Cy is a trademark of GE Healthcare.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
564844 Rev. 2
Antibody Details
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M3/84

The M3/84 monoclonal antibody specifically binds to CD107b which is also known as Mac-3, Lysosome-associated membrane protein 2 (LAMP-2/Lamp2/Lamp II), and Lysosomal membrane glycoprotein type B (LGP-B). CD107b is a single-pass type I transmembrane glycoprotein that constitutes a major integral membrane protein of lysosomes and may play a role in lysosomal function. CD107b is also expressed on the surface of mouse mononuclear phagocytes. Surface expression of the 92-110-kDa glycoprotein antigen increases during differentiation of monocytes to activated macrophages and may play a role in adhesion. The M3/84 mAb can detect CD107b antigen on tissue macrophages, thioglycollate-elicited peritoneal macrophages, and some myeloid cell lines, but not on lymphocytes or monocytes. In the bone marrow, very few cells display CD107b antigen on the surface, but a large proportion express cytoplasmic CD107b. The M3/84 antibody has also been reported to stain dendritic cells and endothelium in sections of thymus (both medulla and cortex), lymph nodes, spleen (white pulp), and gut-associated lymphoid tissue.

        

564844 Rev. 2
Format Details
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PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
564844 Rev.2
Citations & References
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Development References (7)

  1. Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985; 101(1):85-95. (Clone-specific: Immunoprecipitation). View Reference
  2. Flotte TJ, Springer TA, Thorbecke GJ. Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets. Am J Pathol. 1983; 111(1):112-124. (Clone-specific: Immunohistochemistry). View Reference
  3. Ho MK, Springer TA. Tissue distribution, structural characterization, and biosynthesis of Mac-3, a macrophage surface glycoprotein exhibiting molecular weight heterogeneity. J Biol Chem. 1983; 285(1):636-642. (Clone-specific: Immunoprecipitation). View Reference
  4. Ralph P, Ho MK, Litcofsky PB, Springer TA. Expression and induction in vitro of macrophage differentiation antigens on murine cell lines. J Immunol. 1983; 130(1):108-114. (Biology). View Reference
  5. Springer TA. Cell-surface differentiation in the mouse. Characterization of "jumping" and "lineage" antigens using xenogeneic rat monoclonal antibodies. In: Kennett RH, McKearn TJ, Bechtol KB, ed. Monoclonal antibodies. Hybridomas: A new dimension in biological analyses. New York and London: Plenum Press; 1980:185-217.
  6. Springer TA. Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J Biol Chem. 1981; 256(8):3833-3839. (Immunogen: Immunoprecipitation). View Reference
  7. Walker EB, Akporiaye ET, Warner NL, Stewart CC. Characterization of subsets of bone marrow-derived macrophages by flow cytometry analysis. J Leukoc Biol. 1985; 37(2):121-136. (Biology). View Reference
View All (7) View Less
564844 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.