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    PE Rat Anti-Mouse IL-33R (ST2)
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    Consider BD Horizon RealYellow™ 586 (RY586) Reagents, a bright and clean fluorochrome alternative to PE off the yellow-green laser. RY586 can be used alongside PE on spectral cytometers. More Info #
    PE Rat Anti-Mouse IL-33R (ST2)
    Multicolor flow cytometric analysis of IL-33R (ST2) expression by mouse splenocytes. BALB/c mouse splenic leucocytes were stained with BD Horizon™ Fixable Viability Stain 510 (Cat. No. 564406) followed by staining with Alexa Fluor® 488 Rat Anti-Mouse CD4 antibody (Cat. No. 557667) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Mouse IL-33R (ST2) antibody (Cat. No. 566311/566312; Right Plot). The cells were then fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with Alexa Fluor® 647 Rat Anti-Mouse Foxp3 (Cat. No. 560401/560402). Two-color flow cytometric contour plots showing the correlated expression of Foxp3 versus IL-33R (ST2) [or Ig Isotype control staining] were derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
    PE Rat Anti-Mouse IL-33R (ST2)
    Multicolor flow cytometric analysis of IL-33R (ST2) expression by mouse splenocytes. BALB/c mouse splenic leucocytes were stained with BD Horizon™ Fixable Viability Stain 510 (Cat. No. 564406) followed by staining with Alexa Fluor® 488 Rat Anti-Mouse CD4 antibody (Cat. No. 557667) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Mouse IL-33R (ST2) antibody (Cat. No. 566311/566312; Right Plot). The cells were then fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with Alexa Fluor® 647 Rat Anti-Mouse Foxp3 (Cat. No. 560401/560402). Two-color flow cytometric contour plots showing the correlated expression of Foxp3 versus IL-33R (ST2) [or Ig Isotype control staining] were derived from CD4-positive gated events with the forward and side light-scatter characteristics of live cell-discriminated intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
    Product Details
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    BD Pharmingen™
    Il1rl1; ST2; ST2L; IL-33R alpha; IL-33Rα; IL33RA; Ly84; DER4; Fit-1
    Mouse (QC Testing)
    Rat IgG2a, κ
    Mouse IL-33R Recombinant Protein
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    17082
    AB_2744490
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    566312 Rev. 1
    Antibody Details
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    U29-93

    The U29-93 monoclonal antibody specifically binds to the mouse Interleukin-33 Receptor (IL-33 Receptor, or IL-33R) which is also known as ST2. The IL-33R exists in either a type I transmembrane or soluble glycoprotein form. These IL-33R forms are encoded by the Il1rl1 (Interleukin-1 receptor-like 1) gene which belongs to the IL-1 Receptor family within the Ig superfamily. The IL-33R is expressed by subsets of T cells, including Th2-like cells and some regulatory T cells, as well as some innate lymphocytes, eosinophils, basophils, and mast cells. The IL-33R (also known as the IL-33R alpha subunit, or IL-33Rα) binds IL-33 and complexes with the IL-1R Accessory Protein (IL1RAP) to form a functional signaling receptor complex that can induce the production of T helper type 2 (Th2) cytokines. The soluble IL-33R may function as a decoy receptor which can block the binding of IL-33 to the transmembrane IL-33R. The IL-33R plays roles in inflammation, immunity, and allergy.

            

    566312 Rev. 1
    Format Details
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    PE
    R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    PE
    Yellow-Green 488 nm, 532 nm, 561 nm
    496 nm, 566 nm
    576 nm
    566312 Rev.1
    Citations & References
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    Development References (3)

    1. Hayakawa H, Hayakawa M, Kume A, Tominaga S. Soluble ST2 blocks interleukin-33 signaling in allergic airway inflammation.. J Biol Chem. 2007; 282(36):26369-80. (Biology). View Reference
    2. Matta BM, Lott JM, Mathews LR, et al. IL-33 is an unconventional Alarmin that stimulates IL-2 secretion by dendritic cells to selectively expand IL-33R/ST2+ regulatory T cells.. J Immunol. 2014; 193(8):4010-20. (Biology). View Reference
    3. Schiering C, Krausgruber T, Chomka A, et al. The alarmin IL-33 promotes regulatory T-cell function in the intestine.. Nature. 2014; 513(7519):564-8. (Biology). View Reference
    566312 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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