PE Rat Anti-Mouse CD68
Clone FA/11 (also known as FA-11, FA11) (RUO)
- Brand BD Pharmingen™
- Alternative Name Cd68; Macrosialin; Lamp4; gp110; Scard1
- Concentration 0.2 mg/ml
- Isotype Rat IgG2a, κ
- Reactivity Mouse (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
Flow cytometry (Tested During Development)
- Immunogen Purified Con A Acceptor Glycoproteins from the Mouse P815 Cell Line
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The FA/11 monoclonal antibody specifically binds to CD68 which is also known as Macrosialin, Lamp4 or Scard1. CD68 is a heavily glycosylated ~ 85-115 kDa type I transmembrane protein that belongs to the lysosomal-associated membrane protein (LAMP) family. Although it is expressed at the cell surface, it is predominantly expressed as an intracellular protein in late endosomes. CD68 is expressed by macrophages, Kupffer cells, histiocytes, microglia, and is considered a useful pan-macrophage marker. It is also expressed by dendritic cells, Langerhans cells and osteoclasts. CD68 expression is upregulated and its glycosylation patterns can be changed by proinflammatory agents. Although CD68 binds to oxidized low-density lipoproteins, its exact biological function is not well defined.
- Format PE
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
- An isotype control should be used at the same concentration as the antibody of interest.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.