PE Rat Anti-Mouse CD155

This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [753279] or for additional support, contact your local applications specialist. Contact Us

BD Pharmingen™ PE Rat Anti-Mouse CD155

Name: PE Rat Anti-Mouse CD155
Brand: BD Pharmingen™
SKU: 566719

Clone TX56

(RUO)

Multicolor flow cytometric analysis of CD155 expression on mouse thymocytes. C57BL/6 mouse thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Rat Anti-Mouse CD8a (Cat. No. 553031/553030/561966) and APC Rat Anti-Mouse CD4 (Cat. No. 553051/561091) antibodies, and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histograms) or PE Rat Anti-Mouse CD155 antibody (Cat. No. 566719/566720; solid line histograms) at 0.5 μg/test. The fluorescence histograms showing CD155 expression (or Ig Isotype control staining) were derived from CD4-CD8+ (Upper Left Panel), CD4+CD8+(Upper Right), CD4-CD8- (Lower Left), or CD4+CD8- (Lower Right) gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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Product Details

Brand: BD Pharmingen™

Alternative Name: CD155; Pvr; D7Ertd458e; PVS; Taa1; Tage4; HVED; necl-5

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG2a, κ

Immunogen: Mouse CD155 Transfected Cell Line

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 52118

RRID: AB_2744291

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.1 mg Cat No. 553141

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.5 mg Cat No. 553142

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Rat IgG2a, κ Isotype Control RUO

Size 0.1 mg Cat No. 553930

PE Rat Anti-Mouse CD155 RUO

Size 25 µg Cat No. 566720

View Details

566719 Rev. 1

Antibody Details

TX56

The TX56 monoclonal antibody specifically recognizes CD155, which is also known as Poliovirus Receptor (PVR), Tumor-associated glycoprotein E4 (Tage4), or Tumor-associated antigen 1 (Taa1). CD155 is a type I transmembrane glycoprotein that belongs to the Ig supergene family. CD155 is an adhesion receptor that binds to different ligands including nectin-3, CD96 (Tactile), CD226 (DNAM-1), TIGIT, and the extracellular matrix protein vitronectin. It is highly expressed on double-positive thymocytes and variably expressed on mature thymocytes and T cells, including regulatory T cells and NKT cells. CD155 is also differentially expressed on subsets of B cells, plasma cells, dendritic cells, and monocytes. CD155 expression is upregulated by activated T cells, B cells, and dendritic cells. CD155 is involved in forming adherens junctions between adjacent epithelial or endothelial cells. CD155 plays roles in regulating cell growth, adhesion, motility, migration as well as NK and T cell-mediated cytotoxicity.

566719 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

566719 Rev.1

Citations & References

Development References (7)

Danisch S, Qiu Q, Seth S, et al. CD226 interaction with CD155 impacts on retention and negative selection of CD8 positive thymocytes as well as T cell differentiation to follicular helper cells in Peyer's Patches.. Immunobiology. 2013; 218(2):152-8. (Biology). View Reference
Iguchi-Manaka A, Kai H, Yamashita Y, et al. Accelerated tumor growth in mice deficient in DNAM-1 receptor. J Exp Med. 2008; 205(13):2959-2964. (Clone-specific: Flow cytometry). View Reference
Iguchi-Manaka A, Okumura G, Kojima H, et al. Increased Soluble CD155 in the Serum of Cancer Patients. PLoS One. 2016; 11(4):e0152982. (Clone-specific: ELISA). View Reference
Kourepini E, Paschalidis N, Simoes DC, Aggelakopoulou M, Grogan JL, Panoutsakopoulou V. TIGIT Enhances Antigen-Specific Th2 Recall Responses and Allergic Disease. J Immunol. 2016; 196(9):3570. (Clone-specific: Flow cytometry). View Reference
Lenac Rovis T, Kucan Brlic P, Kaynan N, et al. Inflammatory monocytes and NK cells play a crucial role in DNAM-1-dependent control of cytomegalovirus infection. J Exp Med. 2016; 213(9):1835-1850. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
Stanietsky N, Rovis TL, Glasner A, et al. Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR.. Eur J Immunol. 2013; 43(8):2138-50. (Biology). View Reference
Tahara-Hanaoka S, Shibuya K, Onoda Y et al. Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD112). Int Immunol. 2006; 16(4):533-538. (Biology). View Reference

View All (7)

566719 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.