BD Horizon™ PE-CF594 Hamster Anti-Mouse CD152
Clone UC10-4F10-11 (also known as UC10-4F10; 4F10)
(RUO)
Two-color flow cytometric analysis of CD152 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 72 hours with concanavalin A (Con A). The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with APC Rat Anti-Mouse CD8a antibody (Cat. No. 553035/561093) and either BD Horizon™ PE-CF594 Hamster IgG1, κ Isotype Control (Cat. No . 562307; Left Plot) or BD Horizon PE-CF594 Hamster Anti-Mouse CD152 antibody (Cat. No. 564332; Right Plot). Two-color flow cytometric dot plots showing the correlated expression of CD152 (or Ig Isotype control staining) versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable activated leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
.png){kind=spacer}
Two-color flow cytometric analysis of CD152 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 72 hours with concanavalin A (Con A). The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with APC Rat Anti-Mouse CD8a antibody (Cat. No. 553035/561093) and either BD Horizon™ PE-CF594 Hamster IgG1, κ Isotype Control (Cat. No . 562307; Left Plot) or BD Horizon PE-CF594 Hamster Anti-Mouse CD152 antibody (Cat. No. 564332; Right Plot). Two-color flow cytometric dot plots showing the correlated expression of CD152 (or Ig Isotype control staining) versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable activated leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Horizon™
Ctla4; CTLA-4; Cytotoxic T-lymphocyte-associated protein 4; Ly-56
Mouse (QC Testing)
Armenian Hamster IgG1, κ
Mouse CTLA-4 IgG2a Fusion
Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
0.2 mg/ml
12477
AB_2732917
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
- When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
- CF™ is a trademark of Biotium, Inc.
- This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For U.S. patents that may apply, see bd.com/patents.
564332 Rev. 2
Antibody Details
UC10-4F10-11
The UC10-4F10-11 monoclonal antibody specifically binds to CD152, which is also known as Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4). CD152 is expressed on activated T lymphocytes 2-3 days after stimulation through T cell receptor. CTLA-4 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression appears to be restricted to activated T cells and CD25+CD4+ regulatory T (Treg) cells. Whereas CD28 delivers a costimulatory signal required for T-cell activation, CTLA-4 is a negative regulator of cell-mediated immune responses. CD152 may play roles in induction and/or maintenance of immunological tolerance, regulation of protective immunity, and autoimmune responses, and regulation of some aspects of thymocyte maturation. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leucocytes.
564332 Rev. 2
Format Details
PE-CF594
BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE-CF594
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
615 nm
564332 Rev.2
Citations & References
Development References (11)
-
Alegre ML, Noel PJ, Eisfelder BJ, et al. Regulation of surface and intracellular expression of CTLA4 on mouse T cells. J Immunol. 1996; 157(11):4762-4770. (Clone-specific: Flow cytometry, Fluorescence microscopy, Immunofluorescence, Intracellular Staining/Flow Cytometry). View Reference
-
Cilio CM, Daws MR, Malashicheva A, Sentman CL, Holmberg D. Cytotoxic T lymphocyte antigen 4 is induced in the thymus upon in vivo activation and its blockade prevents anti-CD3-mediated depletion of thymocytes. J Exp Med. 1998; 188(7):1239-1246. (Clone-specific: Blocking, Intracellular Staining/Flow Cytometry). View Reference
-
Issazadeh S, Zhang M, Sayegh MH, Khoury SJ.. Acquired thymic tolerance: role of CTLA4 in the initiation and maintenance of tolerance in a clinically relevant autoimmune disease model. J Immunol. 1999; 162(2):761-765. (Clone-specific: In vivo exacerbation). View Reference
-
Kearney ER, Walunas TL, Karr RW, et al. Antigen-dependent clonal expansion of a trace population of antigen-specific CD4+ T cells in vivo is dependent on CD28 costimulation and inhibited by CTLA-4. J Immunol. 1995; 155(3):1032-1036. (Clone-specific: In vivo exacerbation). View Reference
-
Lee KM, Chuang E, Griffin M, et al. Molecular basis of T cell inactivation by CTLA-4. Science. 1998; 282(5397):2263-2266. (Clone-specific: Immunoprecipitation). View Reference
-
McCoy K, Camberis M, Gros GL. Protective immunity to nematode infection is induced by CTLA-4 blockade. J Exp Med. 1997; 186(2):183-187. (Clone-specific: In vivo exacerbation). View Reference
-
Perkins D, Wang Z, Donovan C, et al. Regulation of CTLA-4 expression during T cell activation. J Immunol. 1996; 156(11):4154-4159. (Clone-specific: Flow cytometry). View Reference
-
Perrin PJ, Maldonado JH, Davis TA, June CH, Racke MK. CTLA-4 blockade enhances clinical disease and cytokine production during experimental allergic encephalomyelitis. J Immunol. 1996; 157(4):1333-1336. (Clone-specific: In vivo exacerbation). View Reference
-
Read S, Malmstrom V, Powrie F. Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation. J Exp Med. 2000; 192(2):295-302. (Clone-specific: Intracellular Staining/Flow Cytometry). View Reference
-
Takahashi T, Tagami T, Yamazaki S, et al. Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4. J Exp Med. 2000; 192(2):303-309. (Clone-specific: Flow cytometry, In vivo exacerbation). View Reference
-
Walunas TL, Lenschow DJ, Bakker CY, et al. CTLA-4 can function as a negative regulator of T cell activation.. Immunity. 1994; 1(5):405-13. (Immunogen: Flow cytometry, Immunoprecipitation, Stimulation). View Reference
564332 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
