FITC Mouse Anti-Mouse I-A[k]

This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [562014] or for additional support, contact your local applications specialist. Contact Us

BD Pharmingen™ FITC Mouse Anti-Mouse I-A[k]

Name: FITC Mouse Anti-Mouse I-A[k]
Brand: BD Pharmingen™
SKU: 553536

Clone 11-5.2

(RUO)

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Product Details

Brand: BD Pharmingen™

Alternative Name: Aα[k]; H2-IA[k] alpha chain; H-2 I-A[k] alpha chain

Reactivity: Mouse (QC Testing)

Isotype: Mouse BALB/c IgG2b, κ

Immunogen: CKB mouse splenocytes

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_394905

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

FITC Mouse IgG2b, κ Isotype Control RUO

Size 0.25 mg Cat No. 559532

553536 Rev. 13

Antibody Details

11-5.2

The 11-5.2 monoclonal antibody specifically recognizes the α chain of the I-A[k] MHC class II alloantigen. It cross-reacts with cells from mice of the H-2[r] haplotype. mAb 11-5.2 recognizes the Ia.19 determinant, and it's reactivity is dependent upon the presence of Glu at position 75 of the Aα chain. Reactivity with other H-2 haplotypes (e.g., b, d, f, p, q, s) has not been observed.

553536 Rev. 13

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

553536 Rev.13

Citations & References

Development References (4)

Devaux CA, Phillips ML, Delovitch TL. Idiotypic analysis of anti-I-Ak monoclonal antibodies. II. Detection of shared idiotopes on syngeneic BALB/c and allogeneic A.TH-derived anti-I-Ak mAb by BALB/c-derived anti-I-Ak anti-idiotypic mAb. J Immunol. 1984; 133(5):2595-2602. (Biology). View Reference
Landais D, Marchetto S, Waltzinger C, Pierres M, Benoist C, Mathis D. Slot-machine mutagenesis of a polymorphic residue on the A kappa alpha-chain. J Immunol. 1988; 141(2):667-671. (Biology). View Reference
Landias D, Beck BN, Buerstedde JM, et al.. The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants. J Immunol. 1986; 137(9):3002-3005. (Biology). View Reference
Oi VT, Jones PP, Goding JW, Herzenberg LA, Herzenberg LA. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978; 81:115-129. (Immunogen). View Reference

View All (4)

553536 Rev. 13

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.