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BV421 Rat Anti-Mouse CD169
BV421 Rat Anti-Mouse CD169
Two-color flow cytometric analysis of CD169 expression on mouse myeloid cells. Bone marrow leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse Ly-6C and Ly-6G (Gr-1) antibody (Cat. No. 553129/561083) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Plot) or BD Horizon BV421 Rat Anti-Mouse CD169 antibody (Cat. No. 566604/566606; Right Plot) at 1 µg/test. A two-color flow cytometric dot plot showing the correlated expression pattern of Ly-6C and Ly-6G (Gr-1) versus CD169 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of CD169 expression on mouse myeloid cells. Bone marrow leucocytes from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse Ly-6C and Ly-6G (Gr-1) antibody (Cat. No. 553129/561083) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Plot) or BD Horizon BV421 Rat Anti-Mouse CD169 antibody (Cat. No. 566604/566606; Right Plot) at 1 µg/test. A two-color flow cytometric dot plot showing the correlated expression pattern of Ly-6C and Ly-6G (Gr-1) versus CD169 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Horizon™
Anti-mouse sialoadhesin
Mouse (QC Testing)
Rat AO IgG2a, κ
Purified sialoadhesin from mouse spleen
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739759
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566606 Rev. 1
Antibody Details
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3D6/CD169

The 3D6 monoclonal antibody specifically recognizes CD169 which is a ~185 kDa type I transmembrane glycoprotein that is encoded by Siglec1. CD169 belongs to the Sialic acid binding Ig like lectins (Siglec) family and is also known as Sialoadhesin (Sn), Siglec-1, or Sheep erythrocyte receptor (SER). CD169 is expressed on subsets of macrophages found in the bone marrow, secondary lymphoid tissues, liver colon and lungs. It binds to the oligosaccharide sequence NeuAc alpha 2,3Gal present on glycolipids and glycoproteins including CD43, CD162, CD206, or CD227. CD169 serves as an adhesion molecule that can mediate cellular interactions with the extracellular matrix or with other cells including lymphocytes and myeloid cells. CD169 may play a role in erythroid and myeloid cell development as well as leucocyte trafficking and inflammation.

566606 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
566606 Rev.1
Citations & References
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Development References (4)

  1. Crocker PR, Freeman S, Gordon S, Kelm S. Sialoadhesin binds preferentially to cells of the granulocytic lineage.. J Clin Invest. 1995; 95(2):635-43. (Immunogen: Blocking, Radioimmunoassay). View Reference
  2. Ducreux J, Crocker PR, Vanbever R. Analysis of sialoadhesin expression on mouse alveolar macrophages.. Immunol Lett. 2009; 124(2):77-80. (Clone-specific: Blocking, Inhibition). View Reference
  3. Kumamoto Y, Higashi N, Denda-Nagai K, et al. Identification of sialoadhesin as a dominant lymph node counter-receptor for mouse macrophage galactose-type C-type lectin 1.. J Biol Chem. 2004; 279(47):49274-80. (Clone-specific: Fluorescence microscopy, Immunofluorescence, Western blot). View Reference
  4. Nath D, van der Merwe PA, Kelm S, Bradfield P, Crocker PR. The amino-terminal immunoglobulin-like domain of sialoadhesin contains the sialic acid binding site. Comparison with CD22.. J Biol Chem. 1995; 270(44):26184-91. (Clone-specific: Functional assay, Immunoaffinity chromatography). View Reference
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566606 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.