Skip to main content Skip to navigation
PE Mouse Anti-ROR2
Alert icon
This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
PE Mouse Anti-ROR2
Flow cytometric analysis of ROR2 expression in human chronic myelogenous leukemia cell line. K562 cells (ATCC CCL-243) were fixed in BD Cytofix™ fixation buffer (Cat. No. 554655) and permeabilized in BD Perm/Wash™ buffer (Cat. No. 554723). Permeablized cells were stained with either PE Mouse IgG1, k Isotype Control (Cat. No. 554680, dashed line) or PE Mouse Anti-ROR2 (Cat. No. 565551, solid line). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto™ II Cell Analyzer.
Flow cytometric analysis of ROR2 expression in human chronic myelogenous leukemia cell line. K562 cells (ATCC CCL-243) were fixed in BD Cytofix™ fixation buffer (Cat. No. 554655) and permeabilized in BD Perm/Wash™ buffer (Cat. No. 554723). Permeablized cells were stained with either PE Mouse IgG1, k Isotype Control (Cat. No. 554680, dashed line) or PE Mouse Anti-ROR2 (Cat. No. 565551, solid line). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto™ II Cell Analyzer.
Product Details
Down Arrow Up Arrow


BD Pharmingen™
Neurotrophic tyrosine kinase, receptor-related 2; NTRKR2, BDB, BDB1
Human (QC Testing), Rat (Tested in Development), Mouse (Reported)
Mouse IgG1, κ
Mouse ROR2 Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739292
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  6. An isotype control should be used at the same concentration as the antibody of interest.
565551 Rev. 1
Antibody Details
Down Arrow Up Arrow
Ror2

The Ror2 monoclonal antibody specifically binds to the ST2 intracellular domain of ROR2 (Receptor tyrosine kinase-like Orphan Receptor 2). ROR2 is a type I transmembrane protein that belongs to a small family of receptor tyrosine kinases containing frizzled domains. Based on the homology of ROR2 and Wnt receptors Frz domains, ROR2 has been linked to Wnt signaling and embryonic development. Wnt5a-ROR2 signaling promotes osteoclastogenesis, and ROR2 is also critical for the formation of skeleton, heart, and genitals. In normal adult tissues, ROR2 is expressed in very low levels or undetectable. There is mounting evidence that ROR2 expression is associated with either tumor progression or suppression in different tissues. In humans, mutations in the ROR2 gene are associated with the autosomal recessive form of  Robinow Syndrome and autosomal dominant Brachydactyly type B.

565551 Rev. 1
Format Details
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565551 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Ford CE, Qian Ma SS, Quadir A, Ward RL. The dual role of the novel Wnt receptor tyrosine kinase, ROR2, in human carcinogenesis. Int J Cancer. 2013; 133(4):779-787. (Biology). View Reference
  2. Mikels A, Minami Y, Nusse R. Ror2 receptor requires tyrosine kinase activity to mediate Wnt5A signaling. J Biol Chem. 2009; 284(44):30167-30176. (Immunogen: Immunohistochemistry, Western blot). View Reference
  3. van Amerongen R, Fuerer C, Mizutani M, Nusse R. Wnt5a can both activate and repress Wnt/β-catenin signaling during mouse embryonic development. Dev Biol. 2012; 369(1):101-114. (Clone-specific: Immunohistochemistry, Western blot). View Reference
565551 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.