Description
The Ror2 monoclonal antibody specifically binds to the ST2 intracellular domain of ROR2 (Receptor tyrosine kinase-like Orphan Receptor 2). ROR2 is a type I transmembrane protein that belongs to a small family of receptor tyrosine kinases containing frizzled domains. Based on the homology of ROR2 and Wnt receptors Frz domains, ROR2 has been linked to Wnt signaling and embryonic development. Wnt5a-ROR2 signaling promotes osteoclastogenesis, and ROR2 is also critical for the formation of skeleton, heart, and genitals. In normal adult tissues, ROR2 is expressed in very low levels or undetectable. There is mounting evidence that ROR2 expression is associated with either tumor progression or suppression in different tissues. In humans, mutations in the ROR2 gene are associated with the autosomal recessive form of Robinow Syndrome and autosomal dominant Brachydactyly type B.
Format
- Format
PE
- Excitation Source
Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max
496 nm
- Emission Max
578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species testing during development may have been performed with a different format of the same clone. Selected applications have been tested for cross-reactivity.
- An isotype control should be used at the same concentration as the antibody of interest.
