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PE Mouse Anti-Human ROR1
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Consider BD Horizon RealYellow™ 586 (RY586) Reagents, a bright and clean fluorochrome alternative to PE off the yellow-green laser. RY586 can be used alongside PE on spectral cytometers. More Info #
PE Mouse Anti-Human ROR1
ROR1 expression in human embryonal carcinoma cells. Human NCCIT cells (ATCC CRL-2073) were stained with either PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; dashed line histogram) or PE Mouse Anti-Human ROR1 (Cat. No. 564474; solid-line histogram) monoclonal antibodies. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable NCCIT cells. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
ROR1 expression in human embryonal carcinoma cells. Human NCCIT cells (ATCC CRL-2073) were stained with either PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; dashed line histogram) or PE Mouse Anti-Human ROR1 (Cat. No. 564474; solid-line histogram) monoclonal antibodies. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable NCCIT cells. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
Product Details
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BD Pharmingen™
Neurotrophic tyrosine kinase, receptor-related 1; NTRKR1
Human (QC Testing)
Mouse BALB/c IgG2b, κ
Human ROR1 cDNA
Flow cytometry (Routinely Tested)
5 µl/test
AB_2738822
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
564474 Rev. 1
Antibody Details
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4A5

The 4A5 monoclonal antibody binds to the glycosylated type I membrane protein ROR1 (Receptor-tyrosine-kinase-like Orphan Receptor 1). The ROR1 and ROR2 related proteins are highly conserved evolutionarily and are primarily expressed during embryogenesis. ROR1 is not expressed on normal peripheral blood lymphocytes. ROR1 is similar to tropomyosin receptor kinase (Trk family) neurotropic receptors and shares a cysteine-rich domain with Frizzled receptors for Wnt-family signaling proteins, which are involved in the regulation of embryogenesis and carcinogenesis. Furthermore, there is mounting evidence that aberrant ROR1 expression contributes to human malignancy.

564474 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
564474 Rev.1
Citations & References
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Development References (5)

  1. Broome HE, Rassenti LZ, Wang HY, Meyer LM, Kipps TJ. ROR1 is expressed on hematogones (non-neoplastic human B-lymphocyte precursors) and a minority of precursor-B acute lymphoblastic leukemia. Leuk Res. 2011; 35(10):1390-1394. (Clone-specific: Flow cytometry). View Reference
  2. Cui B, Zhang S, Chen L, Yu J, et al. Targeting ROR1 inhibits epithelial-mesenchymal transition and metastasis. Cancer Res. 2013; 73(12):3649-3660. (Biology). View Reference
  3. Fukuda T, Chen L, Endo T, et al. Antisera induced by infusions of autologous Ad-CD154-leukemia B cells identify ROR1 as an oncofetal antigen and receptor for Wnt5a. Proc Natl Acad Sci U S A. 2008; 105(8):3047. (Immunogen: Flow cytometry). View Reference
  4. Zhang S, Chen L, Cui B, et al. ROR1 is expressed in human breast cancer and associated with enhanced tumor-cell growth. PLoS ONE. 7(3)(Clone-specific: Immunohistochemistry). View Reference
  5. Zhang S, Chen L, Wang-Rodriguez J, et al. The onco-embryonic antigen ROR1 is expressed by a variety of human cancers. Am J Pathol. 2012; 181(6):1903-1910. (Clone-specific: Flow cytometry, Immunocytochemistry (cytospins), Immunohistochemistry, Immunoprecipitation). View Reference
View All (5) View Less
564474 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.