Alexa Fluor® 647 Mouse Anti-Human CD288 (TLR8)
Clone 44C143 (RUO)
- Brand BD Pharmingen™
- Alternative Name TLR8; Toll-like receptor 8
- Concentration 0.2 mg/ml
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
- Immunogen Human TLR8 Peptide
- Workshop No. HCDM 2006
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 44C143 monoclonal antibody specifically binds to CD288 which is also known as the Toll-like receptor 8 (TLR8). CD288 is a type 1 transmembrane glycoprotein that belongs to the Toll-like receptor (TLR) family whose members play fundamental roles in pathogen recognition and activation of inflammatory and immune responses. CD288 is expressed in the endosomal and lysosomal compartments of neutrophils, monocytes, macrophages and dendritic cells. CD288 binds single-stranded microbial RNA and is involved in triggering leucocyte responses against bacteria, and single-stranded or double-stranded RNA viruses. Ligand-bound CD288 triggers the MyD88/NF-κB signal pathway leading to antimicrobial cellular responses including the release of proinflammatory cytokines such as TNF, IL-12, and MIP-1α/CCL3.
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.