PE Mouse Anti-Human Galectin-9
Clone 9M1-3 (RUO)
- Brand BD Pharmingen™
- Alternative Name galectin 9; Gal-9; Ecalectin; HUAT; LGALS9; LEG9; Tumor antigen HOM-HD-21
- Vol. Per Test 5 µl
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
Flow cytometry (Reported)
- Immunogen Human Galectin-9 (AA 208-215) Peptide
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 9M1-3 monoclonal antibody specifically binds to Galectin-9 (Gal-9) which is encoded by LGALS9 (lectin, galactoside-binding, soluble, 9). Galectin-9 is differentially expressed within or on the surface of various cell types including lymphocytes, macrophages, astrocytes, mast cells, eosinophils, fibroblasts, and endothelial cells. Its expression can be induced by cells in response to proinflammatory cytokines or infection by certain viruses. Galectin-9 is an ~ 50 kDa S-type lectin that has two carbohydrate recognition domains (CRDs). It binds to β-galactosides and can serve as a ligand for TIM-3 (CD366). Cell-surface or released forms of Galectin-9 can play multiple roles in innate and adaptive immune responses including the activation of macrophage cytokine secretion, promotion of dendritic cell maturation and regulatory T cell expansion, and negative regulation of Th1, Th17, NK, and cytotoxic T cells. Galectin-9 may contribute to the survival and growth of some tumors by suppressing anti-tumor immune responses. The 9M1-3 antibody reportedly binds to the CRD2 domain of Galectin-9 and can block its binding to TIM-3.
- Format PE
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.