Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Middle East / Africa (English)
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
    Alert Icon
    PE Mouse Anti-Human CD133
    PE Mouse Anti-Human CD133
    CD133 expression on human WERI-Rb-1cells      Panel 1. Cells from the WERI-Rb-1 (Retinoblastoma, ATCC HTB-169) cell line were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human CD133 antibody (Cat. No. 566593/566594; solid line histogram) at 0.06 μg/test. The fluorescence histogram showing CD133 expression (or Ig Isotype control staining) was derived from gated events with forward and side light-scatter characteristics of viable cells. CD133 expression on human peripheral blood mononuclear cells      Panel 2A. Human peripheral blood mononuclear cells (PBMC) were stained with APC Mouse Anti-Human CD34 antibody (Cat. No. 340667) and either PE Mouse IgG1, κ Isotype Control (Left Plot) or PE Mouse Anti-Human CD133 (Right Plot) at 0.06 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells before analysis. Pseudocolor dot plots showing the correlated expression of CD133 (or Ig Isotype control staining) versus CD34 were derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD negative) peripheral blood lymphocytes (PBL).      Panel 2B. Human PBMC were similarly stained with BD Horizon™ BUV395 Mouse Anti-Human CD45 antibody (Cat. No. 563791/563792) and either PE Mouse IgG1, κ Isotype Control (Left Plot) or PE Mouse Anti-Human CD133 antibody (Right Plot) at 0.06 μg/test followed by Cell Viability 7-AAD Solution addition before analysis. Pseudocolor dot plots showing the correlated expression of CD45 versus CD133 (or Ig Isotype control staining) were similarly derived for viable PBL.      Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
    PE Mouse Anti-Human CD133
    CD133 expression on human WERI-Rb-1cells      Panel 1. Cells from the WERI-Rb-1 (Retinoblastoma, ATCC HTB-169) cell line were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human CD133 antibody (Cat. No. 566593/566594; solid line histogram) at 0.06 μg/test. The fluorescence histogram showing CD133 expression (or Ig Isotype control staining) was derived from gated events with forward and side light-scatter characteristics of viable cells. CD133 expression on human peripheral blood mononuclear cells      Panel 2A. Human peripheral blood mononuclear cells (PBMC) were stained with APC Mouse Anti-Human CD34 antibody (Cat. No. 340667) and either PE Mouse IgG1, κ Isotype Control (Left Plot) or PE Mouse Anti-Human CD133 (Right Plot) at 0.06 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells before analysis. Pseudocolor dot plots showing the correlated expression of CD133 (or Ig Isotype control staining) versus CD34 were derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD negative) peripheral blood lymphocytes (PBL).      Panel 2B. Human PBMC were similarly stained with BD Horizon™ BUV395 Mouse Anti-Human CD45 antibody (Cat. No. 563791/563792) and either PE Mouse IgG1, κ Isotype Control (Left Plot) or PE Mouse Anti-Human CD133 antibody (Right Plot) at 0.06 μg/test followed by Cell Viability 7-AAD Solution addition before analysis. Pseudocolor dot plots showing the correlated expression of CD45 versus CD133 (or Ig Isotype control staining) were similarly derived for viable PBL.      Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
    Product Details
    Down Arrow Up Arrow


    BD Pharmingen™
    PROM1; PROML1; prominin-1; AC133; CORD12; MCDR2; MSTP061; RP41; STGD4
    Human (QC Testing)
    Mouse BALB/c IgG1, κ
    WERI-RB-1 Retinoblastoma Cell
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    VII 70485
    8842
    AB_2744281
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
    Show More blue down arrow Show Less blue up arrow

    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    Show More blue down arrow Show Less blue up arrow
    Antibody Details
    Down Arrow Up Arrow
    W6B3C1

    The W6B3C1 monoclonal antibody specifically recognizes CD133 which is also known as Prominin-like protein 1 (PROML1), Prominin-1 (PROM1), hProminin, Hematopoietic stem cell antigen, Macular dystrophy retinal 2 (MCDR2), Stargardt disease 4 autosomal dominant (STGD4), or AC133 antigen. CD133 is an ~120 kDa five-transmembrane, glycoprotein that is encoded by PROM1 (Prominin 1) which belongs to the Prominin gene family. This single-chain, pentaspan transmembrane glycoprotein is comprised of an extracellular N-terminus with two short intracellular sequences and two long extracellular loops followed by an intracellular C-terminus. CD133 is expressed on some cells found in a variety of tissues including the bone marrow, cord and peripheral blood, placenta, liver, pancreas, kidney, lung, retina, brain and heart. It is expressed on various cell types including hematopoietic stem cells and progenitor cells, neural stem cells, developing epithelial cells, precursor endothelial cells, and retinal cells. CD133 is expressed on some cancer cells found in leukemias, melanoma and retinoblastoma. It may serve as a cancer stem cell marker in a number of brain tumors, melanoma, colon cancer, hepatocellular carcinoma, pancreatic adenocarcinoma, and prostate cancer. A mutation in PROM1 is reportedly associated with a form of human retinal degeneration. The W6B3C1 antibody recognizes a different epitope than the human CD133-specific 293C3 antibody.

            

    Format Details
    Down Arrow Up Arrow
    PE
    R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    PE
    Yellow-Green 488 nm, 532 nm, 561 nm
    496 nm, 566 nm
    576 nm
    Citations & References
    Down Arrow Up Arrow

    Development References (5)

    1. Bühring HK, Marzer A, Lammers R, Wissinger B. CD133 cluster report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:622-623.
    2. Koerner SP, André MC, Leibold JS, et al. An Fc-optimized CD133 antibody for induction of NK cell reactivity against myeloid leukemia.. Leukemia. 2017; 31(2):459-469. (Clone-specific: Blocking, Flow cytometry). View Reference
    3. Lammers R, Giesert C, Grünebach F, Marxer A, Vogel W, Bühring HJ. Monoclonal antibody 9C4 recognizes epithelial cellular adhesion molecule, a cell surface antigen expressed in early steps of erythropoiesis.. Exp Hematol. 2002; 30(6):537-45. (Immunogen: Flow cytometry). View Reference
    4. Maw MA, Corbeil D, Koch J, et al. A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration.. Hum Mol Genet. 2000; 9(1):27-34. (Biology). View Reference
    5. Miraglia SJ, Buck D. CD133 (AC133). In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:870-872.
    View All (5) View Less

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

    Website Feedback