Flow cytometric analysis of Vδ2 TCR expression on human peripheral blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human Vδ2 TCR (Cat. No. 555738/562088) and PE Mouse Anti-Human CD3 (Cat. No. 555333/561808/561809). Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Fluorescent two-color contour plot was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.
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Flow cytometric analysis of Vδ2 TCR expression on human peripheral blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human Vδ2 TCR (Cat. No. 555738/562088) and PE Mouse Anti-Human CD3 (Cat. No. 555333/561808/561809). Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Fluorescent two-color contour plot was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.
Product Details
BD Pharmingen™
TCR Vδ2
Human (QC Testing)
Mouse IgG1, κ
Human TCR Vδ2 Recombinant Protein
Flow cytometry (Routinely Tested)
0.5 mg/ml
28517
AB_396081
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Lysing Solution 10X Concentrate CE_IVD
Size
100 mL
Cat No.
349202
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
FITC Mouse IgG1, κ Isotype Control RUO
Size
100 Tests
Cat No.
555748
PE Mouse Anti-Human CD3 RUO
Size
100 Tests
Cat No.
555333
562088 Rev. 2
Antibody Details
B6
The B6 monoclonal antibody specifically recognizes the human variable δ2 subunit (Vδ2) of the γ/δ T cell receptor (TCR). The γ/δ TCR is composed of two disulfide linked glycoproteins. The delta chain is approximately 40-60 kDa and is restricted to peripheral blood T cells and thymocytes. The majority of normal peripheral blood γ/δ T cells express a Vγ9[+]/ Vδ2[+] phenotype. The reason for this selection in the T-cell repertoire is not well understood.
562088 Rev. 2
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
562088 Rev.2
Citations & References
Development References (4)
-
Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
-
Breit TM, Wolvers-Tettero IL, van Dongen JJ. Receptor diversity of human T-cell receptor gamma delta expressing cells. Prog Histochem Cytochem. 1992; 26(1-4):182-193. (Biology). View Reference
-
De Libero G, Rocci MP, Casorati G, et al. T cell receptor heterogeneity in gamma delta T cell clones from intestinal biopsies of patients with celiac disease.. Eur J Immunol. 1993; 23(2):499-504. (Immunogen). View Reference
-
Kabelitz D. Function and specificity of human gamma/delta-positive T cells. Crit Rev Immunol. 1992; 11(5):281-303. (Biology). View Reference
562088 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
