BV711 Mouse Anti-Human ULBP-2/5/6
Clone 165903 (RUO)
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- Alternative Name ULBP2/RAET1H; ULBP5/RAET1G; ULBP6/RAET1L
- Concentration 0.2 mg/ml
- Isotype Mouse IgG2a, κ
- Reactivity Human (Tested in Development)
Flow cytometry (Qualified)
- Immunogen Human ULBP-2 Transfected Cell Line
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 165903 monoclonal antibody specifically recognizes UL16-binding protein 2, 5, and 6 (ULBP-2/5/6). UL16 is a human cytomegalovirus (HCMV) glycoprotein that can bind to human ULBP proteins which share homology with MHC class I proteins. ULBP-2 is also known as RAET1H (retinoic acid early transcript), NKG2DL2 (NKG2D ligand 2), and ALCAN-alpha. ULBP-5 is likewise known as RAET1G whereas ULBP-6 is also referred to as RAET1L. The ULBP-2/5/6 proteins possess only the alpha 1 and alpha 2 Ig-like domains and have no capacity to bind peptides or interact with beta 2 microglobulin. All three proteins can be expressed on the cell surface as glycophosphatidylinositol (GPI)-linked forms whereas ULBP2 and ULBP5 also exist as transmembrane-anchored forms. These proteins may be expressed on various cells types during development or cells experiencing stress caused by high proliferative activity, infection, transformation, or exposure to mediators in inflammatory or autoimmune diseases. The ULBP-2/5/6 proteins can bind to NKG2D and thereby transduce activating signals into NK cells and costimulatory signals into activated CD8+ T cells resulting in enhanced cell-mediated cytotoxicity or cytokine production. The expression of ULBP-2/5/6 proteins on infected or tumor cells can play an important role in immune surveillance and homeostasis.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with BD Horizon BV711 under optimal conditions that minimize unconjugated dye and antibody.
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 711 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
- Cy is a trademark of GE Healthcare.
- Alexa Fluor® is a registered trademark of Life Technologies Corporation.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).