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    BV711 Mouse Anti-Human CD217 (IL-17RA)
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    Try the new Anti-Human CD217 antibody: [749612]
    BV711 Mouse Anti-Human CD217 (IL-17RA)
    Multiparameter flow cytometric analysis using BD OptiBuild™ BV711 Mouse Anti-Human CD217 (IL-17RA) antibody (Cat. No. 747939; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 563044; Left Plot). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.
    BV711 Mouse Anti-Human CD217 (IL-17RA)
    Multiparameter flow cytometric analysis using BD OptiBuild™ BV711 Mouse Anti-Human CD217 (IL-17RA) antibody (Cat. No. 747939; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 563044; Left Plot). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.
    Product Details
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    BD OptiBuild™
    IL17RA; IL-17RA; IL17R; interleukin-17 receptor A; IL-17 receptor A; hIL-17R; CANDF5; IMD51
    Human (Tested in Development)
    Mouse IgG1, κ
    Human CD217 (IL-17RA) Recombinant Protein
    Flow cytometry (Qualified)
    0.2 mg/ml
    HCDM 2006
    23765
    AB_2872400
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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    Recommended Assay Procedures

    BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

    For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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    Product Notices

    1. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    4. An isotype control should be used at the same concentration as the antibody of interest.
    5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
    8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
    9. For U.S. patents that may apply, see bd.com/patents.
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    747939 Rev. 2
    Antibody Details
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    133617

    The 133617 monoclonal antibody specifically recognizes CD217 which is also known as Interleukin-17 receptor A (IL-17R or IL-17RA). CD217 is a ~120 kDa single-pass type I transmembrane glycoprotein that is encoded by IL17RA. CD217 (IL-17RA) belongs to the IL-17 receptor family which also includes IL-17 RB, IL-17RC, IL-17RD, and IL-17RE. CD217 (IL-17RA) has a wide tissue distribution and is variably expressed on granulocytes, monocytes, macrophages, dendritic cells (DCs), T cells, B cells, NK cells, smooth muscle cells, endothelial cells, and epithelial cells. Interleukin-17A (IL-17A) or IL-17F homodimers, or IL-17A:IL-17F heterodimers can bind to and signal through functional IL-17 receptor complexes comprised of CD217 (IL-17RA) and IL-17RC subunits. This leads to the cellular release of proinflammatory cytokines including IL-1, IL-6, and TNF, antimicrobial peptides, and chemokines that attract neutrophils. IL-17E/IL-25 binds to and signals through another functional IL-17 receptor complex comprised of CD217 (IL-17RA) and IL-17RB subunits. IL-17E/IL-25 induces type-2 (Th2-like) immune responses and is implicated in asthma. IL-17C binds to IL-17 receptor complexes comprised of CD217 (IL-17RA) and IL-17RE subunits and can stimulate the production of proinflammatory cytokines and antimicrobial peptides.

    747939 Rev. 2
    Format Details
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    BV711
    The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BV711
    Violet 405 nm
    407 nm
    713 nm
    747939 Rev.2
    Citations & References
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    Development References (5)

    1. Ahn SH, Edwards AK, Singh SS, Young SL, Lessey BA, Tayade C. IL-17A Contributes to the Pathogenesis of Endometriosis by Triggering Proinflammatory Cytokines and Angiogenic Growth Factors.. J Immunol. 2015; 195(6):2591-600. (Clone-specific: Flow cytometry). View Reference
    2. Brucklacher-Waldert V, Steinbach K, Lioznov M, Kolster M, Hölscher C, Tolosa E. Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.. J Immunol. 2009; 183(9):5494-501. (Clone-specific: Flow cytometry). View Reference
    3. Gaffen SL. Structure and signalling in the IL-17 receptor family.. Nat Rev Immunol. 2009; 9(8):556-67. (Biology). View Reference
    4. Yao Z, Spriggs MK, Derry JM, et al. Molecular characterization of the human interleukin (IL)-17 receptor. Cytokine. 1997; 9(11):794-800. (Biology). View Reference
    5. Zola H, Swart B, Banham A, et al. CD molecules 2006--human cell differentiation molecules.. J Immunol Methods. 2007; 319(1-2):1-5. (Clone-specific: Flow cytometry). View Reference
    View All (5) View Less
    747939 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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