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BV650 Rat Anti-Human CD366 (TIM-3)
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Try the new Anti-Human CD366 antibody: [565564]
Product Details
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BD OptiBuild™
CD366; HAVCR2; T cell immunoglobulin mucin 3; TIM3; TIMD-3; TIMD3; KIM-3
Human (Tested in Development)
Rat IgG2a, κ
Human TIM-3 (Ser22-Arg200) Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
X 10-24
AB_2872421
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV650 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 650 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
  10. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
747960 Rev. 1
Antibody Details
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344823

The 344823 monoclonal antibody specifically recognizes CD366, which is also known as T cell immunoglobulin mucin 3 (TIM-3), or T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3/TIMD3). CD366 (TIM-3) is a ~60 kDa type I transmembrane glycoprotein that is encoded by HAVCR2 (Hepatitis A virus cellular receptor 2) and belongs to the human TIM family (along with TIM-1 and TIM-4) within the Ig superfamily. CD366 (TIM-3) has one Ig-like V-type domain and a mucin stalk region which is rich in serine and threonine. It is expressed on type-1 (Th1-like) CD4+ T cells and CD8+ T cells, type-17 (Th17-like) CD4+ T cells, regulatory T cells (Treg), NKT, and NK cells. It is also expressed on dendritic cells, mast cells, monocytes, macrophages, and microglia but not on B cells or type-2 (Th2-like) CD4+ T cells. CD366 (TIM-3) can regulate macrophage activation and suppress type-1 and type-17 T cell functions. Crosslinking of cell surface CD366 (TIM-3) by binding to Galectin-9 and/or phosphatidylserine can either positively or negatively regulate leucocyte functions, such as cytokine production or the phagocytosis of apoptotic cells. Through its suppressive activity, CD366 (TIM-3) helps maintain peripheral immune tolerance and homeostasis, but can also inhibit anti-tumor immunity.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm.  BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter).  Due to the excitation and emission characteristics of the acceptor dye, there will be  spillover into the APC and Alexa Fluor® 700 detectors.  However, the spillover can be corrected through compensation as with any other dye combination.

747960 Rev. 1
Format Details
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BV650
The BD Horizon Brilliant Violet™ 650 (BV650) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 406-nm and an acceptor dye with an emission maximum (Em Max) at 649-nm. BV650, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 650-nm (e.g., a 660/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
BV650
Violet 405 nm
406 nm
649 nm
747960 Rev.1
Citations & References
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Development References (4)

  1. Cherkassky L, Morello A, Villena-Vargas J, et al. Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition.. J Clin Invest. 2016; 126(8):3130-44. (Clone-specific: Flow cytometry). View Reference
  2. Monney L1, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease.. Nature. 2002; 415(6871):536-541. (Biology). View Reference
  3. Mujib S, Jones RB, Lo C, et al. Antigen-independent induction of Tim-3 expression on human T cells by the common γ-chain cytokines IL-2, IL-7, IL-15, and IL-21 is associated with proliferation and is dependent on the phosphoinositide 3-kinase pathway.. J Immunol. 2012; 188(8):3745-56. (Clone-specific: Flow cytometry). View Reference
  4. Ndhlovu LC, Lopez-Verges S, Barbour JD, et al. Tim-3 marks human natural killer cell maturation and suppresses cell-mediated cytotoxicity. Blood. 2012; 119(16):3734-3743. (Clone-specific: Functional assay). View Reference
View All (4) View Less
747960 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.