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BV650 Mouse Anti-Human TCR γδ
BV650 Mouse Anti-Human TCR γδ
Two-color flow cytometric analysis of TCR γδ expressed on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon™ BV650 Mouse Anti-Human TCR γδ (Cat No. 564156) and PE Mouse Anti-Human CD3 (Cat. No. 555333/561808/561809) antibodies. Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202).  The two-color flow cytometric contour plot showing the correlated expression of TCR γδ versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Two-color flow cytometric analysis of TCR γδ expressed on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon™ BV650 Mouse Anti-Human TCR γδ (Cat No. 564156) and PE Mouse Anti-Human CD3 (Cat. No. 555333/561808/561809) antibodies. Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202).  The two-color flow cytometric contour plot showing the correlated expression of TCR γδ versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
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BD Horizon™
TCRgd; γδ TCR; TRG@, TRD@; TCRG, TCRD; TCR gamma delta
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738628
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV650 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV650 were removed.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349).

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
  7. BD Horizon Brilliant Violet 650 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
564156 Rev. 2
Antibody Details
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B1

The B1 monoclonal antibody specifically binds to the γδ T cell receptor (γδ TCR). This receptor complex consists of two disulfide-linked transmembrane glycoproteins, a γ chain (45-60 kDa) and a δ subunit (40-60 kDa). The γδ TCR is associated with the signal-transducing CD3 complex. The γδ TCR is expressed by thymocytes and by peripheral T cell subsets (γδ T cells) that are located in the blood, liver, skin and various lymphoid and mucosal tissues. γδ T cells contribute to both innate and adaptive immune responses to infections and tumors. Reports suggest that γδ T cells may also play roles in antigen presentation and the regulation of autoimmune responses.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm.  BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be  spillover into the APC and Alexa Fluor® 700 detectors.  However, the spillover can be corrected through compensation as with any other dye combination.

564156 Rev. 2
Format Details
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BV650
The BD Horizon Brilliant Violet™ 650 (BV650) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 406-nm and an acceptor dye with an emission maximum (Em Max) at 649-nm. BV650, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 650-nm (e.g., a 660/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV650
Violet 405 nm
406 nm
649 nm
564156 Rev.2
Citations & References
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Development References (7)

  1. Bai L, Picard D, Anderson B, et al. The majority of CD1d-sulfatide-specific T cells in human blood use a semiinvariant Vδ1 TCR.. Eur J Immunol. 2012; 42(9):2505-10. (Clone-specific). View Reference
  2. Bonneville M, O'Brien RL, Born WK. Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity. Nat Rev Immunol. 2110; 10(7):467-478. (Biology). View Reference
  3. Davodeau F, Peyrat MA, Houde I, et al. Surface expression of two distinct functional antigen receptors on human gamma delta T cells. Science. 1993; 260(5115):1800-1802. (Biology). View Reference
  4. De Libero G. Sentinel function of broadly reactive human gamma delta T cells. Immunol Today. 1997; 18(1):22-26. (Biology). View Reference
  5. Del Porto P, D'Amato M, Fiorillo MT, Tuosto L, Piccolella E, Sorrentino R. Identification of a novel HLA-B27 subtype by restriction analysis of a cytotoxic gamma delta T cell clone.. J Immunol. 1994; 153(7):3093-100. (Biology). View Reference
  6. Fitzgerald KA, Callard RE. The cytokine factsbook., 2nd ed. / Katherine A. Fitzgerald ... [et al.].. San Diego: Academic Press; 2001:1-515.
  7. Kabelitz D. γδ T-cells: cross-talk between innate and adaptive immunity. Cell Mol Life Sci. 2011; 68(14):2331-2333. (Biology). View Reference
View All (7) View Less
564156 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.