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    BV421 Mouse Anti-Human NKG2A (CD159a)
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    If you're planning to order more than 10 BD OptiBuild™ Reagents, please contact your local sales representative to place this order. Contact Us #
    BV421 Mouse Anti-Human NKG2A (CD159a)
    Two-color flow cytometric analysis of NKG2A (CD159a) expression on peripheral blood lymphocytes. Peripheral blood mononuclear cells (PBMC) were costained with APC Mouse Anti-Human CD56 (Cat. No. 555518) and either BD Horizon™ BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439; Left Plot) or BD OptiBuild™ BV421 Mouse Anti-Human NKG2A (CD159a) (Cat. No. 747924; Right Plot) at 0.5 μg/test. The pseudocolor dot plots showing the correlated expression of NKG2A (CD159a) [or Ig Isotype control staining] versus CD56 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. The above is qualification data only and does not represent a specific OptiBuild™ lot.
    BV421 Mouse Anti-Human NKG2A (CD159a)
    Two-color flow cytometric analysis of NKG2A (CD159a) expression on peripheral blood lymphocytes. Peripheral blood mononuclear cells (PBMC) were costained with APC Mouse Anti-Human CD56 (Cat. No. 555518) and either BD Horizon™ BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439; Left Plot) or BD OptiBuild™ BV421 Mouse Anti-Human NKG2A (CD159a) (Cat. No. 747924; Right Plot) at 0.5 μg/test. The pseudocolor dot plots showing the correlated expression of NKG2A (CD159a) [or Ig Isotype control staining] versus CD56 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. The above is qualification data only and does not represent a specific OptiBuild™ lot.
    Product Details
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    BD OptiBuild™
    KLRC1; CD159A; NKG2A; NK cell receptor A; natural killer cell lectin; natural killer group protein 2; C-lectin type II protein
    Human (Tested in Development)
    Mouse IgG2a, κ
    Human NKG2A and CD94 Transfected Cell Line
    Flow cytometry (Qualified)
    0.2 mg/ml
    AB_2872385
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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    Recommended Assay Procedures

    BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

    For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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    Product Notices

    1. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
    2. Researchers should determine the optimal concentration of this reagent for their individual applications.
    3. An isotype control should be used at the same concentration as the antibody of interest.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    6. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
    7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
    8. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
    9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
    10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    747924 Rev. 2
    Antibody Details
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    131411

    The 131411 monoclonal antibody specifically recognizes NKG2A which is also known as NK cell receptor A, Natural killer cell lectin, Natural killer group protein 2, C-lectin type II protein, or CD159 antigen-like family member A (CD159a). NKG2A (CD159a) is encoded by KLRC1 (killer cell lectin like receptor C1) which belongs to the killer cell lectin-like receptor (KLR) family. This type II transmembrane glycoprotein contains a C-type lectin domain in its extracellular region and two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its intracellular region. NKG2A (CD159a) is expressed on NK cells and some T cells. NKG2A (CD159a) forms a disulfide-bonded heterodimer with CD94 that can bind to MHC class I HLA-E molecules on target cells and inhibit NK cell activation.

    The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max near 407 nm and Em Max near 421 nm, BD Horizon BV421 can be excited by the violet laser (405 nm) and detected with a 450/50 nm filter. BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific BlueTM conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue, and BD Horizon V450 cannot be used simultaneously.

    747924 Rev. 2
    Format Details
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    BV421
    The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    BV421
    Violet 405 nm
    407 nm
    423 nm
    747924 Rev.2
    Citations & References
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    Development References (4)

    1. Houchins JP, Yabe T, McSherry C, Bach FH. DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells.. J Exp Med. 1991; 173(4):1017-20. (Biology). View Reference
    2. Loyon R, Picard E, Mauvais O, et al. IL-21-Induced MHC Class II+ NK Cells Promote the Expansion of Human Uncommitted CD4+ Central Memory T Cells in a Macrophage Migration Inhibitory Factor-Dependent Manner.. J Immunol. 2016; 197(1):85-96. (Clone-specific: Flow cytometry). View Reference
    3. Messal N, Mamessier E, Sylvain A, et al. Differential role for CD277 as a co-regulator of the immune signal in T and NK cells.. Eur J Immunol. 2011; 41(12):3443-54. (Clone-specific: Functional assay). View Reference
    4. Picardi A, Mengarelli A, Marino M, et al. Up-regulation of activating and inhibitory NKG2 receptors in allogeneic and autologous hematopoietic stem cell grafts.. J Exp Clin Cancer Res. 2015; 34:98. (Clone-specific: Flow cytometry). View Reference
    View All (4) View Less
    747924 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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