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BV421 Mouse Anti-Human CD179a (VpreB)
BV421 Mouse Anti-Human CD179a (VpreB)
Flow cytometric analysis of surface CD179a (VpreB) expression on Human NALM-6 cells. Cells from the Human NALM-6 (B cell precursor leukemia) cell line were surface stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No.562438; dashed line histogram) or BD Horizon BV421 Mouse Anti-Human CD179a (VpreB) antibody (Cat. No. 566583; solid line histogram) at 1.0 μg/test. The fluorescence histogram showing CD179a (VpreB) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of surface CD179a (VpreB) expression on Human NALM-6 cells. Cells from the Human NALM-6 (B cell precursor leukemia) cell line were surface stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No.562438; dashed line histogram) or BD Horizon BV421 Mouse Anti-Human CD179a (VpreB) antibody (Cat. No. 566583; solid line histogram) at 1.0 μg/test. The fluorescence histogram showing CD179a (VpreB) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Horizon™
CD179a; IGI; IGVPB; VPREB; VPREB1; VpreB; VpreB1; pre-B lymphocyte 1; v(pre)B protein
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human VpreB/λ5 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
VII 70514
7441
AB_2739748
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  9. For U.S. patents that may apply, see bd.com/patents.
566583 Rev. 2
Antibody Details
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HSL96

The HSL96 monoclonal antibody specifically recognizes CD179a which is also known as VpreB (V pre beta/V pre β). CD179a (VpreB1) is a ~18 kDa, Ig V domain-like protein that is encoded by VPREB1 (pre-B lymphocyte 1). This member of the immunoglobulin (Ig) gene superfamily is primarily expressed in the cytoplasm of normal pro-B and early pre-B cells and at low levels on the surface of early pre-B cells.  It is not expressed by normal mature circulating B cells or by other leucocyte populations. CD179a (VpreB1) associates noncovalently with CD179b (λ5) to form CD179a/CD179b (VpreB/ λ5), an Ig light chain-like structure called the surrogate light chain. Surrogate light chains are disulfide-linked to membrane-bound IgM heavy chains in association with signal transducer CD79a/CD79b heterodimers which together form the pre-B cell receptor (preBCR) complex. The preBCR plays a critical role in early B cell proliferation and differentiation. The HSL96 antibody can reportedly be used to stain cytoplasmic and cell surface CD179a (VpreB1).

566583 Rev. 2
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
566583 Rev.2
Citations & References
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Development References (6)

  1. Karasuyama H, Lebien TW, Copper MD, Clark EC. CD179 Workshop report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:112-115.
  2. Kiyokawa N, Sekino T, Matsui T, et al. Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma.. Mod Pathol. 2004; 17(4):423-9. (Clone-specific: Flow cytometry). View Reference
  3. Matsuo Y, Drexler HG, Okochi A, Sugimoto A, Harashima A, Orita K. Characterization of human B cell-precursor leukemia and mature B-cell leukaemia/lymphoma cell lines: Expression and distribution of human pre-B-cell receptor. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:117-120.
  4. Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Expression profile of pre B-cell receptor components in acute lymphoblastic leukemia and its application to the diagnosis and classification of the disease. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:115-117.
  5. Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Flow cytometric diagnosis of the cell lineage and developmental stage of acute lymphoblastic leukemia by novel monoclonal antibodies specific to human pre-B-cell receptor.. Blood. 1998; 92(11):4317-24. (Immunogen: ELISA, Flow cytometry, Immunoprecipitation). View Reference
  6. Zola H, Swart B, Boumsell L, Mason DY. Human Leucocyte Differentiation Antigen nomenclature: update on CD nomenclature. Report of IUIS/WHO Subcommittee.. J Immunol Methods. 2003; 275(1-2):1-8. (Clone-specific: Flow cytometry). View Reference
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566583 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.