Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239.
Companion Products
The 767329 monoclonal antibody specifically recognizes Sialic acid-binding Ig-like lectin 6 (Siglec-6) which is also known as Obesity-binding protein 1 (OB-BP1), CD33 antigen-like 1 (CD33L1), or CD327. Siglec-6 (CD327) is a type I transmembrane glycoprotein that is encoded by SIGLEC6 which belongs to the Sialic acid-binding Ig-like lectins (Siglecs) superfamily. Siglec-6 (CD327) has one N-terminal Ig-like V-set domain followed by two Ig-like C2-type domains in its extracellular region. Its intracellular region has an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as a signaling lymphocyte activating molecule (SLAM)-like motif that can serve as a docking site for SAP (SLAM-associated protein). This I-type lectin can also exist in a soluble form of the extracellular region that lacks the transmembrane and cytoplasmic regions. Siglec-6 (CD327) is expressed on placental trophoblasts and B cells and at low levels on neutrophils. It specifically recognizes sialyl Tn-containing molecules and binds to leptin and is involved in cellular interactions and signaling.
The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.
Development References (3)
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Botting RA, Bertram KM, Baharlou H, et al. Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes.. J Leukoc Biol. 2017; 101(6):1393-1403. (Clone-specific: Flow cytometry). View Reference
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Madge PD, Maggioni A, Pascolutti M, et al. Structural characterisation of high affinity Siglec-2 (CD22) ligands in complex with whole Burkitt's lymphoma (BL) Daudi cells by NMR spectroscopy.. Sci Rep. 2016; 6:36012. (Clone-specific: Flow cytometry). View Reference
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Takei Y, Sasaki S, Fujiwara T, Takahashi E, Muto T, Nakamura Y. Molecular cloning of a novel gene similar to myeloid antigen CD33 and its specific expression in placenta.. Cytogenet Cell Genet. 1997; 78(3-4):295-300. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
