BUV395 Mouse Anti-Human CD107b
Clone H4B4 (RUO)
- Brand BD Horizon™
- Alternative Name LAMP-2; LAMP2; LAMPB; LGP110
- Vol. Per Test 5 µl
- Isotype Mouse IgG1, κ
- Reactivity Human (QC Testing)
- Application
Intracellular staining (flow cytometry) (Routinely Tested)
- Immunogen Human Adult Adherent Peripheral Blood Cells
- Workshop No. V P007
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Description
The H4B4 monoclonal antibody specifically binds to CD107b. CD107b is a heavily glycosylated, ~120 kDa type I transmembrane protein that is also known as lysosomal-associated membrane protein 2 (LAMP-2/LAMP2), LAMPB, or LGP110. CD107b is expressed in the lysosomal and endosomal membranes of most cell types where it may play a role in maintaining membrane integrity. CD107b is also expressed on the surface of activated platelets and lymphocytes, including T lymphocytes, e.g., degranulating CD8+ cytotoxic T cells. CD107b serves as a ligand for selectins, including E-selectin, and mediates cellular adhesion. CD107a/ LAMP-1 and CD107b /LAMP-2 are carriers for poly-N-acetyllactosamines, and display the sialyl Le[x] antigen. CD107b is likewise expressed on some tumor cells and cell lines, including U937 and KG1a, and may facilitate tumor metastases.
The antibody was conjugated to BD Horizon BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355 nm laser equipped instrument. With an Ex Max at 348 nm and an Em Max at 395 nm, this dye has virtually no spillover into any other detector. BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.
Format
BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355-nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.
Suggested Companion Products
Brilliant Stain Buffer RUO
100 Tests
Cat No: 563794
BUV395 Mouse IgG1, k Isotype Control RUO
50 µg
Cat No: 563547
Fixation Buffer RUO
100 mL
Cat No: 554655
Perm/Wash Buffer RUO
100 mL
Cat No: 554723
Stain Buffer (FBS) RUO
500 mL
Cat No: 554656
Stain Buffer (BSA) RUO
500 mL
Cat No: 554657
Resources & Tools | ||||||
---|---|---|---|---|---|---|
Spectrum Viewer | Panel Designer | Spectrum Viewer | Regulatory Document Website |
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with BD Horizon BUV395 under optimum conditions, and unconjugated antibody and free BD Horizon BUV395 were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.