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Biotin Rat IgM, κ Isotype Control RUO

Biotin Rat IgM, κ Isotype Control 

Clone  R4-22   (RUO)

  • Brand BD Pharmingen™
  • Vol. Per Test 20 µl
  • Isotype Rat IgM, κ
  • Application Isotype control, Flow cytometry (Routinely Tested) 
  • Immunogen Pooled Mouse Ig
  • Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The R4-22 monoclonal antibody has a Rat IgM, κ isotype and has no known specificity.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Format
  • Format Biotin

Other Formats →

Suggested Companion Products

PE Streptavidin RUO
0.5 mg Cat No: 554061

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.


An isotype control should be used at the same concentration as the antibody of interest.


  1. Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. View reference

559941 Rev. 5
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