BB700 Rat Anti-Human CD366 (TIM-3)

Try the new Anti-Human CD366 antibody: [746178]

BD OptiBuild™ BB700 Rat Anti-Human CD366 (TIM-3)

Name: BB700 Rat Anti-Human CD366 (TIM-3)
Brand: BD OptiBuild™
SKU: 747957

Clone 344823

(RUO)

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Product Details

Brand: BD OptiBuild™

Alternative Name: CD366; HAVCR2; T cell immunoglobulin mucin 3; TIM3; TIMD-3; TIMD3; KIM-3

Reactivity: Human (Tested in Development)

Isotype: Rat IgG2a, κ

Immunogen: Human TIM-3 (Ser22-Arg200) Recombinant Protein

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

Workshop Number: X 10-24

RRID: AB_2872418

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BB700 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

This antibody was developed for use in flow cytometry.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Researchers should determine the optimal concentration of this reagent for their individual applications.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
BD Horizon Brilliant Blue 700 is covered by one or more of the following US patents: 8,455,613 and 8,575,303.
Cy is a trademark of GE Healthcare.

Companion Products

Human BD Fc Block™ RUO

Size 50 µg Cat No. 564219

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Brilliant Stain Buffer RUO

Size 100 Tests Cat No. 563794

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Lysing Solution 10X Concentrate CE_IVD

Size 100 mL Cat No. 349202

View Details

747957 Rev. 1

Antibody Details

344823

The 344823 monoclonal antibody specifically recognizes CD366, which is also known as T cell immunoglobulin mucin 3 (TIM-3), or T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3/TIMD3). CD366 (TIM-3) is a ~60 kDa type I transmembrane glycoprotein that is encoded by HAVCR2 (Hepatitis A virus cellular receptor 2) and belongs to the human TIM family (along with TIM-1 and TIM-4) within the Ig superfamily. CD366 (TIM-3) has one Ig-like V-type domain and a mucin stalk region which is rich in serine and threonine. It is expressed on type-1 (Th1-like) CD4+ T cells and CD8+ T cells, type-17 (Th17-like) CD4+ T cells, regulatory T cells (Treg), NKT, and NK cells. It is also expressed on dendritic cells, mast cells, monocytes, macrophages, and microglia but not on B cells or type-2 (Th2-like) CD4+ T cells. CD366 (TIM-3) can regulate macrophage activation and suppress type-1 and type-17 T cell functions. Crosslinking of cell surface CD366 (TIM-3) by binding to Galectin-9 and/or phosphatidylserine can either positively or negatively regulate leucocyte functions, such as cytokine production or the phagocytosis of apoptotic cells. Through its suppressive activity, CD366 (TIM-3) helps maintain peripheral immune tolerance and homeostasis, but can also inhibit anti-tumor immunity.

747957 Rev. 1

Format Details

BB700

The BD Horizon Brilliant™ Blue 700 (BB700) Dye is part of the BD Horizon Brilliant™ Blue family of dyes. This tandem fluorochrome is comprised of a polymer-technology dye donor with an excitation maximum (Ex Max) of 476-nm and an acceptor dye with an emission maximum (Em Max) at 695-nm. Driven by BD innovation, BB700 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 695-nm (e.g., a 695/20-nm bandpass filter). The donor dye can be excited by the Violet (405 nm) laser and the acceptor dye can be excited by the red (627–640 nm) laser resulting in cross-laser excitation and fluorescence spillover. BB700 Reagents are significantly brighter than equivalent PerCP or PerCP-Cy5.5 reagents and are less sensitive to photobleaching. In addition, BB700 shows much less excitation by the violet (407-nm) laser resulting in less spillover. BB700 has minimal yellow green (562-nm) excitation and is ideal for instruments with both blue (488-nm) and yellow green (562-nm) lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BB700

Excitation Source: Blue 488 nm

Excitation Max: 476 nm

Emission Max: 695 nm

747957 Rev.1

Citations & References

Development References (4)

Cherkassky L, Morello A, Villena-Vargas J, et al. Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition.. J Clin Invest. 2016; 126(8):3130-44. (Clone-specific: Flow cytometry). View Reference
Monney L1, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease.. Nature. 2002; 415(6871):536-541. (Biology). View Reference
Mujib S, Jones RB, Lo C, et al. Antigen-independent induction of Tim-3 expression on human T cells by the common γ-chain cytokines IL-2, IL-7, IL-15, and IL-21 is associated with proliferation and is dependent on the phosphoinositide 3-kinase pathway.. J Immunol. 2012; 188(8):3745-56. (Clone-specific: Flow cytometry). View Reference
Ndhlovu LC, Lopez-Verges S, Barbour JD, et al. Tim-3 marks human natural killer cell maturation and suppresses cell-mediated cytotoxicity. Blood. 2012; 119(16):3734-3743. (Clone-specific: Functional assay). View Reference

View All (4)

747957 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.