Flow cytometric analysis of CD61 expression on human peripheral blood platelets. Platelets were isolated from fresh human whole blood and fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing, the fixed platelets were stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; dashed line histogram) or APC Mouse Anti-Human CD61 antibody (Cat. No. 564174; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
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Flow cytometric analysis of CD61 expression on human peripheral blood platelets. Platelets were isolated from fresh human whole blood and fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing, the fixed platelets were stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; dashed line histogram) or APC Mouse Anti-Human CD61 antibody (Cat. No. 564174; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
Integrin β3; Integrin beta-3; GP3A; GPIIIa; ITGB3; ITB3
Human (QC Testing), Rhesus, Cynomolgus, Baboon, Dog, Cow (Tested in Development)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
5 µl
III 866; IV P83; V T-124
3690
AB_2738645
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
APC Mouse IgG1 κ Isotype Control RUO
Size
0.1 mg
Cat No.
554681
564174 Rev. 2
Antibody Details
VI-PL2
The VI-PL2 monoclonal antibody specifically binds to CD61. CD61 is a 105 kDa transmembrane glycoprotein that is also known as integrin β3 and platelet glycoprotein IIIa (GPIIIa or GP3A). It is expressed on platelets, megakaryocytes, osteoclasts and endothelia. Integrin β3 associates with gpIIa (CD41) to form the CD41/CD61 complex which mediates platelet adhesion and aggregation. CD61 also associates with CD51 to form the CD51/CD61 complex (vitronectin receptor). CD61 appears to bind to fibrinogen, fibronectin, vWF, vitronectin, and thrombospondin to mediate cell adhesion.
564174 Rev. 2
Format Details
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
564174 Rev.2
Citations & References
Development References (3)
-
Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
-
Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002.
-
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
564174 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
