APC Mouse Anti-Human CD41a
Clone HIP8 (RUO)
- Brand BD Pharmingen™
- Alternative Name ITGA2B; Integrin alpha-2b (αIIb); Platelet glycoprotein IIb (GPIIb)
- Vol. Per Test 20 µl
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing) Rhesus, Cynomolgus, Baboon (Tested in Development)
Flow cytometry (Routinely Tested)
- Immunogen Purified platelet membrane glycoproteins
- Workshop No. IV P38
- Entrez Gene ID 3674
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The HIP8 monoclonal antibody specifically binds to the α-chain of CD41. CD41 is also known as Integrin αIIb or Platelet GPIIb. The calcium-dependent complex of CD41 and CD61 (β3 integrin or GPIIIa) is normally expressed on platelets and megakaryocytes. The CD41/CD61 complex is the receptor for fibrinogen, fibronectin and von Willebrand factor, and mediates platelet adhesion and aggregation. CD41 (clone HIP8) completely inhibits ADP-, epinephrine-, and collagen-induced platelet activation, and partially inhibits ristocetin- and thrombin-induced platelet activation. This antibody is useful in the morphological and physiological studies of platelets and megakaryocytes.
Clone HIP8 also cross-reacts with a major subset of peripheral blood platelets of baboon, and both rhesus and cynomolgus macaque monkeys. The staining pattern of platelets is similar to that observed with peripheral blood platelets from normal human donors.
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.