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    APC Mouse Anti-Human CD273
    APC Mouse Anti-Human CD273
    Specificity of mAb MIH18 for CD273. The PD-L2-transfected cell line MIH90 was stained with either Mouse IgG1, κ isotype control mAb MOPC-21 (Cat. no. 555751, dashed histogram) or APC mAb MIH18 (solid histogram).  When the PD-1-transfected cell line MIH43 was stained in parallel with the same reagents, no difference was seen between isotype control and anti-CD273 (data not shown).  Flow cytometry was perfomed on a BD FACSCalibur™ flow cytometry system.  
    APC Mouse Anti-Human CD273
    Specificity of mAb MIH18 for CD273. The PD-L2-transfected cell line MIH90 was stained with either Mouse IgG1, κ isotype control mAb MOPC-21 (Cat. no. 555751, dashed histogram) or APC mAb MIH18 (solid histogram).  When the PD-1-transfected cell line MIH43 was stained in parallel with the same reagents, no difference was seen between isotype control and anti-CD273 (data not shown).  Flow cytometry was perfomed on a BD FACSCalibur™ flow cytometry system.  
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    BD Pharmingen™
    PD-1 ligand 2; PD-L2; B7-DC; PDCD1LG2; PDCD1 ligand 2; PDCD1L2; PDL2; Btdc
    Human (QC Testing)
    Mouse IgG1, κ
    Human CD273 Transfected Cell Line
    Flow cytometry (Routinely Tested)
    20 µl
    VIII 80539
    80380
    AB_647162
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
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    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    557926 Rev. 3
    Antibody Details
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    MIH18

    The MIH18 monoclonal antibody specifically binds to CD273 which is also known as the Programmed Death Ligand 2 (PD-L2) and B7-DC. CD273 is a type 1 transmembrane glycoprotein member of the B7 family and Ig superfamily. CD273 serves as a ligand for CD279, the Programmed Death 1 (PD-1) receptor. CD273 is expressed by dendritic cells, activated monocytes, medullary thymic epithelial cells, placental trophoblasts, and myocardial endothelium. CD273 can function as a coinhibitor of T cell functions by acting through CD279. Monoclonal antibodies that block the CD273 and CD279 interaction result in enhanced T cell proliferation and cytokine production. Thus, CD273 can play an important role in regulating T cell responses. Clone MIH18 is reportedly a blocking antibody.

    557926 Rev. 3
    Format Details
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    APC
    Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    APC
    Red 627-640 nm
    651 nm
    660 nm
    557926 Rev.3
    Citations & References
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    Development References (5)

    1. Brown JA, Dorfman DM, Ma FR, et al. Blockade of programmed death-1 ligand on dendritic cells enhances T cell activation and cytokine production. J Immunol. 2003; 170:1257-1266. (Biology). View Reference
    2. Carreno BM, Bennett F, Chau TA, et al. CTLA-4 (CD152) can inhibit T cell activation by two different mechanisms depending on its level of cell surface expression.. J Immunol. 2000; 165(3):1352-6. (Biology). View Reference
    3. Carter L, Fouser LA, Jussif J, et al. PD-1:PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2. Eur J Immunol. 2002; 32:634-643. (Biology). View Reference
    4. Latchman Y, Wood CR, Chernova T, et al. PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2001; 2(3):261-268. (Biology). View Reference
    5. Ohigashi Y, Sho M, Yamada Y, et al. Clinical significance of programmed death-1 ligand-1 and programmed death-1 ligand-2 expression in human esophageal cancer. Clin Cancer Res. 2005; 11:2947-2953. (Immunogen). View Reference
    View All (5) View Less
    557926 Rev. 3

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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