Alexa Fluor® 647 Mouse Anti-Integrin αvβ5

BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Integrin αvβ5

Name: Alexa Fluor® 647 Mouse Anti-Integrin αvβ5
Brand: BD Pharmingen™
SKU: 565836

Clone ALULA

(RUO)

Flow cytometric analysis of Integrin αVβ5 expression on human and mouse cell lines. Cells from the SW480 (Human colon adenocarcinoma, ATCC CCL-228; Left Plot), HT-1080 (Human fibrosarcoma, ATCC CCL-121; Middle Plot), or NIH/3T3 (Mouse embryonic fibroblast, ATCC CRL-1658; Right Plot) cell lines were stained with either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No. 565378; dashed line histograms) or Alexa Fluor® 647 Mouse Anti-Integrin αvβ5 antibody (Cat. No. 565836; solid line histograms). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a FACSCanto™ II Flow Cytometer System. Please note that the cells were maintained at 4°C throughout staining and analysis for optimal results.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Integrin alpha v beta 5; αVβ5; CD51/β5

Reactivity: Human (QC Testing), Mouse (Tested in Development)

Isotype: Mouse 129, also known as 129/J or 129/SvJ IgG2b, κ

Immunogen: Mouse fibroblasts expressing αvβ5

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_2739376

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Recommended Assay Procedures

Investigators: Please note that for optimal results, the target cells should be maintained at 4°C throughout staining and analysis .

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control RUO

Size 50 µg Cat No. 565378

565836 Rev. 1

Antibody Details

ALULA

The ALULA monoclonal antibody specifically binds to Integrin alpha v beta 5 (αvβ5), which is also known as CD51/β5. Integrin αvβ5 is widely expressed by fibroblasts, endothelial cells, and vascular smooth muscle cells. It is involved in activation-dependent cell migration and cell adhesion to the extracellular matrix. Integrin αvβ5 plays a major role in growth factor-induced angiogenesis in response to VEGF and TGF. The αv Integrins, αvβ5 and αvβ3, αre involved in tumor growth and are being targeted in cancer therapies. The ALULA clone specifically recognizes αvβ5 integrin and functionally blocks the interaction between αvβ5 and vitronectin. Although raised against mouse αvβ5, the ALULA antibody crossreacts with human αvβ5.

565836 Rev. 1

Format Details

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: Alexa Fluor™ 647

Excitation Source: Red 627-640 nm

Excitation Max: 653 nm

Emission Max: 669 nm

565836 Rev.1

Citations & References

Development References (3)

Burvenich I, Schoonooghe S, Vervoort L, et al. Monoclonal antibody 14C5 targets integrin alphavbeta5.. Mol Cancer Ther. 2008; 7(12):3771-9. (Biology). View Reference
Lee P, Bax DV, Bilek MM, Weiss AS. A novel cell adhesion region in tropoelastin mediates attachment to integrin αVβ5.. J Biol Chem. 2014; 289(3):1467-77. (Biology). View Reference
Su G1, Hodnett M, Wu N, et al. Am J Respir Cell Mol Biol. 2007; 36(3):377-386. (Immunogen: Blocking, Flow cytometry, Immunoprecipitation, Inhibition). View Reference

565836 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.