Alexa Fluor® 647 Mouse Anti-Human CLEC5A (MDL-1)
Clone 283834 (RUO)
- Brand BD Pharmingen™
- Alternative Name CLEC5A; CLECSF5; MDL1; MDL-1; Myeloid DAP12-associating Lectin 1
- Concentration 0.2 mg/ml
- Isotype Mouse IgG2b, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Human
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Monoclonal antibody 283834 specifically recognizes C-type lectin domain family 5, member A (CLEC5A) which is also known as C-type lectin superfamily member 5 (CLECSF5) or Myeloid DAP12-associating Lectin-1 (MDL-1). CLEC5A (MDL-1) is a ~40 kDa type II transmembrane glycoprotein that is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, and neutrophils. Clec5A (MDL-1) has a charged lysine in its transmembrane region that allows it to associate with DAP12 (aka, TYROBP) and transduce an activating signal in myeloid cells. Clec5A (MDL-1) regulates inflammatory responses during autoimmune arthritis and upon binding to Dengue viruses. The 283834 antibody does not crossreact with recombinant mouse Clec5a (MDL1) protein.
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.