Multiparameter analysis of human CD369 (Clec7A) expression on peripheral blood leucocytes. Whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 565357; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD369 (Clec7A) antibody (Cat. No. 564855; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Contour plots showing the correlated expression patterns of human CD369 (CLEC7A) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Multiparameter analysis of human CD369 (Clec7A) expression on peripheral blood leucocytes. Whole blood was stained with either Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 565357; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD369 (Clec7A) antibody (Cat. No. 564855; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Contour plots showing the correlated expression patterns of human CD369 (CLEC7A) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Pharmingen™
CLEC7A; CLC7A; BGR; CLECSF12; CANDF4;Dectin-1
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human Dectin-1 Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
X 10-79
64581
AB_2738985
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate CE_IVD
Size
100 mL
Cat No.
349202
Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control RUO
Size
50 µg
Cat No.
565357
564855 Rev. 2
Antibody Details
15E2
The 15E2 monoclonal antibody specifically binds to CD369 which is also known as CLEC7A (C-type lectin domain family 7 member A), Dectin-1 (Dendritic cell-associated C-type lectin 1), or BGR (Beta-glucan receptor). CD369 is a type II transmembrane C-type lectin that contains a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain, a transmembrane segment, and an ITAM containing cytoplasmic tail. This protein is highly expressed on dendritic cells. It is also expressed on B cells, monocytes, eosinophils, mast cells, macrophages, endothelial cells, and is lowly expressed on some subsets of T cells. CD369 binds to beta-glucan polymers and functions as a pattern recognition receptor in innate immune response to fungal and bacterial pathogens. The 15E2 antibody can reportedly stimulate dendritic cells to costimulate immune responses.
564855 Rev. 2
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564855 Rev.2
Citations & References
Development References (5)
-
Hermanz-Falcon P, Arce I, Roda-Navarro P, Fernandez-Ruiz E. Cloning of human DECTIN-1, a novel C-type lectin-like receptor gene expressed on dendritic cells. Immunogenetics. 2001; 53(4):288-295. (Biology). View Reference
-
Ni L, Gayet I, Zurawski S, et al. Concomitant activation and antigen uptake via human dectin-1 results in potent antigen-specific CD8+ T cell responses. J Immunol. 2010; 185(6):3504-3513. (Immunogen: ELISA, Flow cytometry, Fluorescence microscopy, Functional assay, Immunofluorescence, Stimulation). View Reference
-
Sobanov Y, Bernreiter A, Derdak S, et al. A novel cluster of lectin-like receptor genes expressed in monocytic, dendritic and endothelial cells maps close to the NK receptor genes in the human NK gene complex. Eur J Immunol. 2001; 31(12):3493-3503. (Biology). View Reference
-
Willment JA, Gordon S, Brown GD. Characterization of the human beta -glucan receptor and its alternatively spliced isoforms. J Biol Chem. 2001; 276(47):43818-43823. (Biology). View Reference
-
Yokota K, Takashima A, Bergstresser PR, Ariizumi K. Identification of a human homologue of the dendritic cell-associated C-type lectin-1, dectin-1. Gene. 2001; 272(1-2):51-60. (Biology). View Reference
564855 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
