Alexa Fluor® 488 Mouse Anti-Human Aiolos

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BD Pharmingen™ Alexa Fluor® 488 Mouse Anti-Human Aiolos

Name: Alexa Fluor® 488 Mouse Anti-Human Aiolos
Brand: BD Pharmingen™
SKU: 565216

Clone S50-895

(RUO)

Multicolor flow cytometric analysis of Aiolos expression in human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. The leucocytes were washed and then stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 555335/561810/561811; Left Panels) and PerCP-Cy™5.5 Mouse Anti-human CD19 antibody (Cat. No. 340951; Right Panels). The cells were subsequently fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (Cat. No. 557721; Lower Panels) or Alexa Fluor® 488 Mouse Anti-Human Aiolos antibody (Cat. No. 565216; Upper Panels). Two-color flow cytometric contour plots showing the correlated expression of Aiolos (or Ig Isotype control staining) versus CD3 or CD19 were derived from gated events with the forward and side-light scattering characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: AIOLOS; AIO; IKZF3; ZNFN1A3

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Human Aiolos Recombinant Protein

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Vol. Per Test: 5 µl

RRID: AB_2739116

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Transcription Factor Buffer Set RUO

Size 100 Tests Cat No. 562574

Alexa Fluor® 488 Mouse IgG1 κ Isotype Control RUO

Size 100 Tests Cat No. 557721

APC Mouse Anti-Human CD3 RUO

Size 100 Tests Cat No. 555335

View Details

565216 Rev. 1

Antibody Details

S50-895

The S50-895 monoclonal antibody specifically recognizes the transcription factor, Aiolos, which is encoded by IKZF3, IKAROS family zinc finger 3. Aiolos belongs to the Ikaros family of zinc-finger proteins that includes Ikaros and Helios. Aiolos can form homodimers or heterodimers with other Ikaros family members. Multiple Aiolos isoforms have been described. Aiolos is expressed in committed lymphoid progenitors and mature types of T and B lymphocytes, but is not expressed by hematopoietic stem cells. Aiolos plays an important role in the control of B lymphocyte differentiation and proliferation and is specifically required for the generation of long-lived, high affinity plasma cells in the bone marrow. Aiolos is involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner. Aberrant expression of IKZF3 has been implicated with leukemogenesis and certain autoimmune diseases.

565216 Rev. 1

Format Details

Alexa Fluor™ 488

Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: Alexa Fluor™ 488

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 517 nm

565216 Rev.1

Citations & References

Development References (8)

Billot K, Parizot C, Arrouss I, et al. Differential aiolos expression in human hematopoietic subpopulations. Leuk Res. 2010; 34(3):289-293. (Biology). View Reference
Billot K, Soeur J, Chereau F, et al. Deregulation of Aiolos expression in chronic lymphocytic leukemia is associated with epigenetic modifications. Blood. 2011; 117(6):1917-1927. (Biology). View Reference
Liippo J, Nera KP, Veistinen E, et al. Both normal and leukemic B lymphocytes express multiple isoforms of the human Aiolos gene. Eur J Immunol. 2001; 31(12):3469-3474. (Biology). View Reference
Morgan B, Sun L, Avitahl N, et al. Aiolos, a lymphoid restricted transcription factor that interacts with Ikaros to regulate lymphocyte differentiation. EMBO J. 1997; 16(8):2004-2013. (Biology). View Reference
Rebollo A, Ayllon V, Fleischer A, Martinez CA, Zaballos A. The association of Aiolos transcription factor and Bcl-xL is involved in the control of apoptosis. J Immunol. 2001; 167(11):6366-6373. (Biology). View Reference
Romero F, Martinez AC, Camonis J, Rebollo A. Aiolos transcription factor controls cell death in T cells by regulating Bcl-2 expression and its cellular localization. EMBO J. 1999; 18(12):3419-3430. (Biology). View Reference
Schmitt C, Tonnelle C, Dalloul A, Chabannon C, Debre P, Rebollo A. Aiolos and Ikaros: regulators of lymphocyte development, homeostasis and lymphoproliferation. Apoptosis. 2002; 7(3):277-284. (Biology). View Reference
Wang JH, Avitahl N, Cariappa A, et al. Aiolos regulates B cell activation and maturation to effector state. Immunity. 1998; 9(4):543-553. (Biology). View Reference

View All (8)

565216 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.