Purified Mouse Anti-GS15
Clone 19/GS15 (RUO)
- Brand BD Transduction Laboratories™
- Alternative Name Golgi SNARE of 15 kDa
- Concentration 250 µg/ml
- Isotype Mouse IgG1
- Reactivity Human (QC Testing) Mouse, Rat, Dog, Frog (Tested in Development)
Western blot (Routinely Tested)
Immunofluorescence (Tested During Development)
- Immunogen Rat GS15 aa. 1-85
- Storage Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
Eukaryotic protein trafficking involves the packaging of target molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Components of both the vesicle and the synaptic plasma membrane interact to form a fusion complex which mediates specific docking and fusion of vesicles. This complex contains NSF (N-ethyl-maleimide-sensitive factor), SNAPs (soluble NSF attachment proteins), and receptor proteins (SNAREs) that include synaptobrevin, synaptotagmin, syntaxin, and SNAP-25 (synaptosome-associated protein of 25 kDa). SNAP-25 and syntaxin are associated with the target plasma membrane (t-SNAREs), while synaptobrevin and synaptotagmin are vesicle-associated proteins (v-SNAREs). In Drosophila, protein transport from the ER to Golgi involves Bet1p, a v-SNARE. With 28% amino acid identity to Bet1p, GS15 (Golgi SNARE of 15 kDa) is widely expressed in rat tissues. It is an integral membrane protein of the Golgi apparatus and functions as a SNARE. Thus, GS15 is thought to be a novel SNARE that participates in ER-Golgi protein transport through an undefined process.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
- Format Purified
Suggested Companion Products
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at -20°C.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml