Alexa Fluor® 647 Mouse Anti-PTF1A
Clone S25-763 (RUO)
- Brand BD Pharmingen™
- Alternative Name PTF1α; Ptf1a; Ptf1α; bHLHa29; PTF1-p48
- Concentration 0.2 mg/ml
- Isotype Mouse IgG1, κ
- Reactivity Mouse (QC Testing) Human, Rat (Tested in Development)
Intracellular staining (flow cytometry) (Routinely Tested)
Immunofluorescence (Tested During Development)
- Immunogen Human PTF1A Recombinant Protein
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The S25-763 monoclonal antibody specifically binds PTF1A (Pancreas specific transcription factor 1a). PTF1A is a transcription factor that contains a basic helix-loop-helix motif domain and, in combination with p75 and p64, forms the pancreas transcription factor complex. PTF1A plays a roles in pancreatic and cerebellar development. In early pancreatic development, pancreatic bud cells can either continue to pancreatic organogenesis or regress to a duodenal fate, and PTF1A plays a role in this differentiation. In late pancreatic development, the expression of PTF1A is restricted to acinar cells where it is involved in the maintenance of exocrine-specific gene expression. PTF1A expression is often evaluated to monitor pancreatic lineage differentiation from pluripotent stem cells. In addition, PTF1A is expressed during retinal development, and mutations in PTF1A cause cerebellar deficiencies.
This mouse anti-human PTF1A monoclonal antibody, clone S25-763, has been demonstrated to cross-react with rat and mouse pancreatic acinar cells.
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Species testing during development may have been performed with a different format of the same clone. Selected applications have been tested for cross-reactivity.
- An isotype control should be used at the same concentration as the antibody of interest.