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R718 Mouse Anti-Human CD61
Product Details
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BD OptiBuild™
Integrin β3; Integrin beta-3; GP3A; GPIIIa; ITGB3; ITB3
Human (Tested in Development)
Mouse BALB/c IgG1
Purified platelet membrane glycoproteins
Flow cytometry (Qualified)
0.2 mg/ml
3690
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Researchers should determine the optimal concentration of this reagent for their individual applications.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
752050 Rev. 1
Antibody Details
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RUU-PL7F12

The RUU-PL7F12 monoclonal antibody specifically recognizes CD61, a 110 kDa type I transmembrane glycoprotein, also known as Glycoprotein IIIa (gpIIIa), the common β-subunit (integrin β3-chain) of the gpIIb/IIIa complex and the vitronectin receptor (VNR). The gpIIb/IIIa complex and the VNR are integrins, ie, α/β-heterodimeric glycoprotein complexes that are involved in cell adhesion. With the CD41 antigen (gpIIb or αIIb), the CD61 antigen forms the gpIIb/IIIa complex, which acts as a receptor for fibrinogen, von Willebrand factor (vWf), fibronectin, and vitronectin on activated platelets. With the CD51 antigen (VNR α-chain or αv), the CD61 antigen forms the VNR, which mediates activation-independent cell adhesion to vitronectin, vWf, fibrinogen, and thrombospondin. The CD61 antigen is found on all normal resting and activated platelets. Platelets from individuals with Glanzmann's thrombasthenia show a >90% reduction of binding of CD61, and heterozygote carriers of the disorder show approximately 50% reduction. The CD61 antigen is also found on endothelial cells, megakaryocytes, and on some myeloid, erythroid, and T-lymphoid leukemic cell lines.

The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.

752050 Rev. 1
Format Details
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R718
The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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R718
Red 627-640 nm
695 nm
718 nm
752050 Rev.1
Citations & References
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Development References (13)

  1. Abrams CS, Ellison N, Budzynski AZ, Shattil SJ. Direct detection of activated platelets and platelet-derived microparticles in humans.. Blood. 1990; 75(1):128-38. (Biology). View Reference
  2. Fijnheer R, Modderman PW, Veldman H, et al. Detection of platelet activation with monoclonal antibodies and flow cytometry. Changes during platelet storage.. Transfusion. 1990; 30(1):20-5. (Biology). View Reference
  3. Fitzgerald LA, Steiner B, Rall SC, Lo SS, Phillips DR. Protein sequence of endothelial glycoprotein IIIa derived from a cDNA clone. Identity with platelet glycoprotein IIIa and similarity to "integrin".. J Biol Chem. 1987; 262(9):3936-9. (Biology). View Reference
  4. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 1992; 69(1):11-25. (Biology). View Reference
  5. Jennings LK, Ashmun RA, Wang WC, Dockter ME. Analysis of human platelet glycoproteins IIb-IIIa and Glanzmann's thrombasthenia in whole blood by flow cytometry.. Blood. 1986; 68(1):173-9. (Biology). View Reference
  6. Modderman PW. CD29/CDw49, CD47, CD51, CD55, and CD61. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1017-1019.
  7. Modderman PW. Cluster report: CD61. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1025.
  8. Parmentier S, Kaplan C, Catimel B, McGregor JL. New families of adhesion molecules play a vital role in platelet functions.. Immunol Today. 1990; 11(7):225-7. (Biology). View Reference
  9. Shattil SJ, Hoxie JA, Cunningham M, Brass LF. Changes in the platelet membrane glycoprotein IIb.IIIa complex during platelet activation.. J Biol Chem. 1985; 260(20):11107-14. (Biology). View Reference
  10. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology). View Reference
  11. Wong DA, Springer TA. CD61 (b3) cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1664-1665. View Reference
  12. von dem Borne AEGKr, Modderman PW, Admiraal LG, Nieuwenhuis, HK. Platelet antibodies, the overall results. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:951-966.
  13. von dem Borne AEGKr, Modderman PW. Cluster report: CD41. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:997-999.
View All (13) View Less
752050 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.