PE Mouse Anti-Human CD298

This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [753525] or for additional support, contact your local applications specialist. Contact Us

BD Pharmingen™ PE Mouse Anti-Human CD298

Name: PE Mouse Anti-Human CD298
Brand: BD Pharmingen™
SKU: 566792

Clone P-3E10

(RUO)

Multiparameter flow cytometric analysis of CD298 expression on human peripheral blood leucocyte populations. Human peripheral blood cells were stained with either Mouse IgG2a, κ Isotype Control (Cat No. 553457, Left Plot) or PE Mouse Anti-Human CD298 antibody (Cat No. 566792, Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The pseudocolor density plot showing the correlated expression of CD298 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Cell Analyzer System and FlowJo™ software.

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Product Details

Brand: BD Pharmingen™

Alternative Name: ATP1B3; ATPB-3; Na, K-ATPase beta-3 polypeptide; CD298

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG2a, κ

Immunogen: Human MOLT-4 Cell Line

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Workshop Number: VIII 80199

RRID: AB_2869870

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Lysing Solution 10X Concentrate CE_IVD

Size 100 mL Cat No. 349202

PE Mouse IgG2a, κ Isotype Control RUO

Size 0.1 mg Cat No. 553457

566792 Rev. 1

Antibody Details

P-3E10

The P-3E10 monoclonal antibody specifically recognizes CD298, the β3 subunit of the Na+/K+ ATPase (Sodium/potassium-transporting ATPase subunit beta-3). CD298 is a ~45-55 kDa single-pass, type II membrane protein that is encoded by ATP1B3 (ATPase Na+/K+ transporting subunit beta 3) which belongs to the P-type ATPases superfamily. CD298 is widely expressed on lymphocytes, monocytes, granulocytes, platelets, and on other hematopoietic and nonhematopoietic cells and cell lines. The Na+/K+ ATPase is an integral membrane protein complex with enzymatic activity that mediates the active transport and exchange of sodium and potassium ions across the plasma membrane. This complex is composed of α and β subunits. The α subunit is a 10-membrane-spanning, catalytic protein that contains binding sites for Na +, K + and ATP. The α subunits are associated with the smaller, regulatory glycoprotein β subunits. Upon ATP hydrolysis, the Na+/K+ ATPase transports Na+ ions out of the cell in exchange for K+ ions that are transported in. This establishes a transmembrane electrochemical gradient that is essential for osmoregulation and for the transport of various nutrients and other molecules by cells. The P-3E10 antibody can reportedly inhibit the activation and proliferation of T cells and B cells.

566792 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

566792 Rev.1

Citations & References

Development References (6)

Chiampanichayakul S, Khunkaewla P, Pata S, Kasinrerk W. Na, K ATPase beta3 subunit (CD298): association with alpha subunit and expression on peripheral blood cells.. Tissue Antigens. 2006; 68(6):509-17. (Clone-specific: Flow cytometry). View Reference
Chiampanichayakul S, Szekeres A, Khunkaewla P, et al. Engagement of Na,K-ATPase beta3 subunit by a specific mAb suppresses T and B lymphocyte activation.. Int Immunol. 2002; 14(12):1407-14. (Immunogen: Flow cytometry, Functional assay, Inhibition). View Reference
Chruewkamlow N, Pata S, Mahasongkram K, Laopajon W, Kasinrerk W, Chiampanichayakul S. β3 subunit of Na,K ATPase regulates T cell activation with no involvement of Na,K ATPase activity.. Immunobiology. 2015; 220(5):634-40. (Clone-specific: Functional assay, Inhibition). View Reference
Horváth O, Drbel K, Angelisová P, Hilgert I, Horejsí V. Non-lineage antigens: section report. Cell Immunol. 2005; 236(1-2):42-47. (Clone-specific). View Reference
Swart B, Salganik MP, Wand MP, et al. The HLDA8 blind panel: findings and conclusions. J Immunol Methods. 2005; 305(1):75-83. (Clone-specific). View Reference
Takheaw N, Laopajon W, Surinkaew S, Khummuang S, Pata S, Kasinrerk W. Ligation of Na, K ATPase beta3 subunit on monocytes by a specific monoclonal antibody mediates T cell hypofunction. PLoS One. 2018; 13(6):e0199717. (Clone-specific: Flow cytometry, Functional assay, Inhibition). View Reference

View All (6)

566792 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.