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    BV421 Rat Anti-Mouse IgG2a
    BV421 Rat Anti-Mouse IgG2a
    Flow cytometric analysis of CD4 expression on rat splenic leucocytes. Rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody (Rat BD Fc Block™) (Cat. No. 550270/550271). The cells were then either not incubated (background control; dashed line histogram) or were incubated with Purified Mouse IgG2a, κ Anti-Rat CD4 antibody (Cat. No. 554835; solid line histogram). The cells were washed and then stained with BD Horizon™ BV421 Rat Anti-Mouse IgG2a antibody (Cat. No. 565817).  The fluorescence histogram showing CD4 expression (or control staining) was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
    BV421 Rat Anti-Mouse IgG2a
    Flow cytometric analysis of CD4 expression on rat splenic leucocytes. Rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody (Rat BD Fc Block™) (Cat. No. 550270/550271). The cells were then either not incubated (background control; dashed line histogram) or were incubated with Purified Mouse IgG2a, κ Anti-Rat CD4 antibody (Cat. No. 554835; solid line histogram). The cells were washed and then stained with BD Horizon™ BV421 Rat Anti-Mouse IgG2a antibody (Cat. No. 565817).  The fluorescence histogram showing CD4 expression (or control staining) was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
    Product Details
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    BD Horizon™
    Ighg2a; Immunoglobulin gamma-2a heavy chain; Igh-1; Igh-1a
    Mouse (QC Testing)
    Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
    Pooled BALB/c and C57BL/6 mouse Ig
    Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
    0.2 mg/ml
    380793
    AB_2739367
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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    Recommended Assay Procedures

    For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    7. An isotype control should be used at the same concentration as the antibody of interest.
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    565817 Rev. 1
    Antibody Details
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    R19-15

    The R19-15 antibody recognizes an epitope in the CH3 domain of mouse IgG2a, with strong reactivity to the Igh-I[a] allotype and weaker reactivity to Igh-I[b]. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] allele, which encodes IgG2a[b], is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.

    565817 Rev. 1
    Format Details
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    BV421
    The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BV421
    Violet 405 nm
    407 nm
    423 nm
    565817 Rev.1
    Citations & References
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    Development References (4)

    1. Buono C, Binder CJ, Stavrakis G, Witztum JL, Glimcher LH, Lichtman AH. T-bet deficiency reduces atherosclerosis and alters plaque antigen-specific immune responses.. Proc Natl Acad Sci USA. 2005; 102(5):1596-601. (Clone-specific: ELISA). View Reference
    2. Justement LB, Kreiger J, Cambier JC. Production of multiple lymphokines by the A20.1 B cell lymphoma after cross-linking of membrane Ig by immobilized anti-Ig.. J Immunol. 1989; 143(3):881-9. (Clone-specific: Activation, Flow cytometry, Functional assay, Stimulation). View Reference
    3. Martin RM, Silva A, Lew AM. The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype. Immunogenetics. 1997; 46(2):167-168. (Biology). View Reference
    4. Morgado MG, Cam P, Gris-Liebe C, Cazenave PA, Jouvin-Marche E. Further evidence that BALB/c and C57BL/6 gamma 2a genes originate from two distinct isotypes. EMBO J. 1989; 8(11):3245-3251. (Biology). View Reference
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    565817 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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