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    BV711 Rat Anti-Mouse IgG1
    BV711 Rat Anti-Mouse IgG1
    Flow cytometric analysis of CD22.2 expression by mouse splenocytes using BD Horizon™ BV711 Rat Anti-Mouse IgG1 as a second step. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either Purified Mouse IgG1, κ Anti-Mouse CD22.2 antibody (clone Cy34.1; solid line histogram) or with no antibody (for background staining control; dashed line histogram). After washing, the cells were stained with BD Horizon BV711 Rat Anti-Mouse IgG1 antibody (Cat. No. 565786) as the second step.  The fluorescence histogram showing CD22.2 expression (or control staining) as detected by BD Horizon BV711 Rat Anti-Mouse IgG1 antibody was derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
    BV711 Rat Anti-Mouse IgG1
    Flow cytometric analysis of CD22.2 expression by mouse splenocytes using BD Horizon™ BV711 Rat Anti-Mouse IgG1 as a second step. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either Purified Mouse IgG1, κ Anti-Mouse CD22.2 antibody (clone Cy34.1; solid line histogram) or with no antibody (for background staining control; dashed line histogram). After washing, the cells were stained with BD Horizon BV711 Rat Anti-Mouse IgG1 antibody (Cat. No. 565786) as the second step.  The fluorescence histogram showing CD22.2 expression (or control staining) as detected by BD Horizon BV711 Rat Anti-Mouse IgG1 antibody was derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
    Product Details
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    BD Horizon™
    Ighg1; Immunoglobulin heavy constant gamma 1; Igh-4
    Mouse (QC Testing)
    Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
    Pooled Mouse IgG1
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    16017
    AB_2739356
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV711 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV711 were removed.
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    Recommended Assay Procedures

    For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    6. Cy is a trademark of Amersham Biosciences Limited.
    7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    8. An isotype control should be used at the same concentration as the antibody of interest.
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    565786 Rev. 1
    Antibody Details
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    A85-1

    The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.

    565786 Rev. 1
    Format Details
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    BV711
    The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BV711
    Violet 405 nm
    407 nm
    713 nm
    565786 Rev.1
    Citations & References
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    Development References (3)

    1. Bhattacharya D, Lee DU, Sha WC. Regulation of Ig class switch recombination by NF-kappaB: retroviral expression of RelB in activated B cells inhibits switching to IgG1, but not to IgE. Int Immunol. 2002; 14(9):983-991. (Clone-specific: Flow cytometry). View Reference
    2. Honjo T, Obata M, Yamawaki-Katoaka Y, et al. Cloning and complete nucleotide sequence of mouse immunoglobulin gamma 1 chain gene. Cell. 1979; 18(2):559-568. (Biology). View Reference
    3. Ozaki K, Spolski R, Ettinger R, et al. Regulation of B cell differentiation and plasma cell generation by IL-21, a novel inducer of Blimp-1 and Bcl-6. J Immunol. 2004; 173(9):5361-5371. (Clone-specific: Flow cytometry). View Reference
    565786 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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